对接种由重组SA11轮状病毒核心样颗粒（CLP）或病毒样颗粒（VLP）组成的亚单位疫苗的奶牛，针对轮状病毒和病毒蛋白的同型特异性抗体反应。
Isotype-specific antibody responses to rotavirus and virus proteins in cows inoculated with subunit vaccines composed of recombinant SA11 rotavirus core-like particles (CLP) or virus-like particles (VLP).
作者信息
Fernandez F M, Conner M E, Parwani A V, Todhunter D, Smith K L, Crawford S E, Estes M K, Saif L J
机构信息
Food Animal Health Research Program, Ohio Agricultural Research and Development Center, Ohio State University, Columbus, USA.
出版信息
Vaccine. 1996 Oct;14(14):1303-12. doi: 10.1016/s0264-410x(96)00065-5.
The isotype antibody responses to bovine IND P5, G6 and simian SA11 P2, G3 rotavirus and SA11 rotavirus proteins (VP4, VP6 and VP7) in serum, colostrum and milk were analysed by ELISA in three groups of vaccinated cows and nonvaccinated controls. Pregnant cows were vaccinated intramuscularly and intramammarily with recombinant baculovirus-expressed SA11 rotavirus VLP (triple-layered virus-like particles containing rotavirus VP2, VP4, VP6 and VP7); CLP (double-layered core-like particles containing rotavirus VP2 and VP6); or inactivated SA11 rotavirus, respectively. Rotavirus antigen titers were highest (30-200-fold) in ELISA in the VLP vaccine compared to the inactivated SA11 vaccine. The IgG1, IgG2 and IgM geometric mean antibody titers (GMT) to rotavirus (titers to bovine rotavirus vs SA11 rotavirus did not differ significantly for any isotype or group) and the IgG2 GMT to VP6 in serum at calving in the vaccinated groups were significantly (P < 0.05) higher than in the control group. In colostrum, IgG1 and IgA rotavirus antibody titers were significantly elevated for VLP (IgG1 GMT 832225; IgA GMT 16384), CLP (IgG1 GMT 660561; IgA GMT 10321) and SA11 (IgG1 GMT 131072; IgA GMT 1448) vaccinated cows compared to control cows (IgG1 GMT 11585; IgA GMT 45). The IgG1 and IgA GMT to rotavirus were significantly elevated (6-100-fold) in milk of VLP and CLP vaccinated cows compared to SA11 vaccinated or control cows. The isotype antibody responses to VP6 in serum, colostrum and milk paralleled the responses to rotavirus, but titers were approximately 2-10-fold lower. Only cows vaccinated with VLP had significantly enhanced serum, colostral and milk antibody titers to rotavirus VP4 and VP7. These results demonstrate that rotavirus antibody titers in serum, colostrum and milk are significantly enhanced by use of non-infectious VLP, CLP and inactivated SA11 rotavirus vaccines, but the VLP or CLP vaccines induced the highest antibody responses, corresponding to their higher rotavirus antigen titers measured by ELISA.
通过酶联免疫吸附测定（ELISA）分析了三组接种疫苗的奶牛和未接种疫苗的对照组血清、初乳和乳汁中针对牛IND P5、G6以及猴SA11 P2、G3轮状病毒和SA11轮状病毒蛋白（VP4、VP6和VP7）的同种型抗体反应。对怀孕奶牛分别进行肌肉注射和乳腺内注射重组杆状病毒表达的SA11轮状病毒VLP（包含轮状病毒VP2、VP4、VP6和VP7的三层病毒样颗粒）、CLP（包含轮状病毒VP2和VP6的双层核心样颗粒）或灭活的SA11轮状病毒。与灭活的SA11疫苗相比，ELISA检测中VLP疫苗的轮状病毒抗原滴度最高（高30 - 200倍）。接种疫苗组产犊时血清中针对轮状病毒的IgG1、IgG2和IgM几何平均抗体滴度（GMT）（针对牛轮状病毒与SA11轮状病毒的滴度在任何同种型或组中均无显著差异）以及针对VP6的IgG2 GMT显著（P < 0.05）高于对照组。在初乳中，接种VLP（IgG1 GMT 832225；IgA GMT 16384）、CLP（IgG1 GMT 660561；IgA GMT 10321）和SA11（IgG1 GMT 131072；IgA GMT 1448）的奶牛的IgG1和IgA轮状病毒抗体滴度显著高于对照奶牛（IgG1 GMT 11585；IgA GMT 45）。与接种SA11或对照奶牛相比，接种VLP和CLP的奶牛乳汁中针对轮状病毒的IgG1和IgA GMT显著升高（6 - 100倍）。血清、初乳和乳汁中针对VP6的同种型抗体反应与针对轮状病毒的反应相似，但滴度低约2 - 10倍。只有接种VLP的奶牛血清、初乳和乳汁中针对轮状病毒VP4和VP7的抗体滴度显著增强。这些结果表明，使用非感染性VLP、CLP和灭活的SA11轮状病毒疫苗可显著提高血清、初乳和乳汁中的轮状病毒抗体滴度，但VLP或CLP疫苗诱导的抗体反应最高，这与ELISA检测到的它们较高的轮状病毒抗原滴度相对应。
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