牛轮状病毒的空斑试验
Plaque assay of bovine rotavirus.
作者信息
Butchaiah G, Lund E
出版信息
Acta Vet Scand. 1983;24(4):362-73. doi: 10.1186/BF03546710.
Abstract
Incorporation of trypsin and diethylaminoethyl-dextran in the overlay was found to be necessary for infectivity assay of the UK strain of bovine rotavirus by plaque assays. Small plaques of about 1 mm in radius were formed in BGM cells. Large plaques of about 3–4 mm in radius were consistently produced in monolayers of secondary calf kidney cultures.
摘要
通过蚀斑试验对英国牛轮状病毒株进行感染性测定时，发现覆盖物中加入胰蛋白酶和二乙氨基乙基葡聚糖是必要的。在BGM细胞中形成了半径约1毫米的小蚀斑。在二代犊牛肾细胞培养单层中始终产生半径约3 - 4毫米的大蚀斑。
相似文献
1
Plaque assay of bovine rotavirus.牛轮状病毒的空斑试验
Acta Vet Scand. 1983;24(4):362-73. doi: 10.1186/BF03546710.
2
Plaque formation of canine rotavirus in cultures of GBK bovine cell line.犬轮状病毒在GBK牛细胞系培养物中的噬菌斑形成
Nihon Juigaku Zasshi. 1988 Feb;50(1):251-3. doi: 10.1292/jvms1939.50.251.
3
A sensitive plaque assay for bovine rotavirus in cultures of the bovine cell line GBK.一种用于在牛细胞系GBK培养物中检测牛轮状病毒的灵敏蚀斑测定法。
Vet Microbiol. 1987 Apr;13(4):383-7. doi: 10.1016/0378-1135(87)90069-1.
4
Development of a rotavirus plaque assay using Sephadex G-75.使用葡聚糖凝胶G-75进行轮状病毒蚀斑试验的开发。
J Virol Methods. 1990 Apr;28(1):25-31. doi: 10.1016/0166-0934(90)90084-s.
5
Susceptibility of bovine rotavirus to interferon. Brief report.
Arch Virol. 1981;70(4):377-9. doi: 10.1007/BF01320253.
6
Comparative study of bovine rotavirus isolates by plaque assay.通过蚀斑试验对牛轮状病毒分离株进行比较研究。
Can J Comp Med. 1984 Jul;48(3):286-9.
7
Comparison by plaque assay of bovine rotavirus and a bovine enterovirus-like agent.通过蚀斑试验对牛轮状病毒和一种牛肠道病毒样因子进行比较。
Vet Microbiol. 1986 Jul;12(2):189-94. doi: 10.1016/0378-1135(86)90080-5.
8
Rotavirus survival and stability in foods as determined by an optimised plaque assay procedure.通过优化的蚀斑测定程序确定轮状病毒在食物中的存活和稳定性。
Int J Food Microbiol. 2000 Nov 1;61(2-3):177-85. doi: 10.1016/s0168-1605(00)00378-0.
9
Weak susceptibility of rotavirus to bovine interferon in calf kidney cells.
Arch Virol. 1980;64(2):167-70. doi: 10.1007/BF01318020.
10
Identification of a bovine rotavirus gene and gene product influencing cellular attachment.鉴定一种影响细胞黏附的牛轮状病毒基因及其基因产物。
J Virol. 1984 Aug;51(2):489-96. doi: 10.1128/JVI.51.2.489-496.1984.
本文引用的文献
1
Genetic recombination for antigenic markers of antigenically different strains of influenza B virus.乙型流感病毒抗原性不同毒株抗原标记的基因重组
J Virol. 1974 Feb;13(2):347-52. doi: 10.1128/JVI.13.2.347-352.1974.
2
Reproducible results with the Gram stain.革兰氏染色结果具有可重复性。
J Pathol Bacteriol. 1962 Jul;84:241-3. doi: 10.1002/path.1700840131.
3
A simplified plaque assay for influenza viruses in Madin-Darby kidney (MDCK) cells.一种用于在马-达二氏犬肾（MDCK）细胞中检测流感病毒的简化蚀斑测定法。
J Virol Methods. 1980;1(2):117-20. doi: 10.1016/0166-0934(80)90020-8.
4
Proteolytic enhancement of rotavirus infectivity: biology mechanism.轮状病毒感染性的蛋白水解增强作用：生物学机制
Virology. 1980 Mar;101(2):432-9. doi: 10.1016/0042-6822(80)90456-0.
5
Enhancement of vaccinia virus plaque formation by trypsin.胰蛋白酶对痘苗病毒蚀斑形成的增强作用。
Proc Soc Exp Biol Med. 1967 Nov;126(2):515-7. doi: 10.3181/00379727-126-32492.
6
Enhancement of reovirus infectivity by extracellular removal or alteration of the virus capsid by proteolytic enzymes.通过蛋白水解酶对病毒衣壳进行细胞外去除或改造来增强呼肠孤病毒的感染性。
J Gen Virol. 1970 Aug;8(2):83-94. doi: 10.1099/0022-1317-8-2-83.
7
Simian virus SA11 and the related O agent.猿猴病毒SA11及相关的O因子。
Arch Gesamte Virusforsch. 1967;22(1):235-45. doi: 10.1007/BF01240518.
8
Cell culture propagation of neonatal calf diarrhea (scours) virus.新生犊牛腹泻病毒的细胞培养增殖
Can Vet J. 1971 Mar;12(3):69-72.
9
Plaque formation by influenza viruses in the presence of trypsin.流感病毒在胰蛋白酶存在的情况下形成噬菌斑。
J Gen Virol. 1974 Dec;25(3):351-7. doi: 10.1099/0022-1317-25-3-351.
10
Factors affecting plaque assay of animal viruses--with special reference to vesicular stomatitis and vaccinia virus.影响动物病毒蚀斑试验的因素——特别以水疱性口炎病毒和痘苗病毒为例
Acta Pathol Microbiol Scand B Microbiol Immunol. 1971;219:Suppl 219:1-69.
Zotero 插件