Beijing National Laboratory for Molecular Sciences, the Key Laboratory of Polymer Chemistry and Physics of the Ministry of Education, College of Chemistry and Molecular Engineering, Peking University, Beijing, PR China.
Electrophoresis. 2010 Jan;31(3):520-7. doi: 10.1002/elps.200900440.
Poor compatibility was the major drawback of polymer mixtures when used as DNA separation media. Using poly(ethylene oxide)-b-poly(N, N-dimethylacrylamide) (PEO(44)-b-PDMA(88)) and PEO (M(w): 1.3 MDa) as an example, we demonstrated the concept that the compatibility was significantly improved when mixing a homopolymer with its copolymer. Laser light scattering indicated that the major interaction between PEO and PEO-b-PDMA in dilute solution was the weak hydrodynamic interaction, which showed almost no effect on the viscosity and the static scattering pattern. In semidilute or concentrated solution, viscosity measurement also suggested good compatibility between the two components. When tested as DNA separation medium by CE, the viscosity of the mixture was extremely low, only 5 cP for 5.0 m/v% PEO-b-PDMA+0.1 m/v% PEO at 25 degrees C. The performance on DNA separation could be tuned by varying the concentration of each component as well as the component ratio. Good separation on both dsDNA and ssDNA was achieved.
聚合物混合物作为 DNA 分离介质时，其主要缺点是相容性差。本文以聚（氧化乙烯）-b-聚（N，N-二甲基丙烯酰胺）（PEO(44)-b-PDMA(88))和 PEO（Mw：1.3 MDa）为例，证明了在混合均聚物与其共聚物时，相容性可以显著提高。激光光散射表明，在稀溶液中 PEO 和 PEO-b-PDMA 之间的主要相互作用是弱的流体力学相互作用，对粘度和静态散射模式几乎没有影响。在半稀或浓溶液中，粘度测量也表明两种组分之间具有良好的相容性。当用作 CE 的 DNA 分离介质时，混合物的粘度极低，在 25°C 时，5.0 m/v% PEO-b-PDMA+0.1 m/v% PEO 的混合物的粘度仅为 5 cP。通过改变每个组分的浓度以及组分比，可以调整 DNA 分离的性能。可以实现对 dsDNA 和 ssDNA 的良好分离。
