Wu C, Liu T, Chu B
Department of Chemistry, State University of New York at Stony Brook, 11794-3400, USA.
Electrophoresis. 1998 Feb;19(2):231-41. doi: 10.1002/elps.1150190216.
The viscosity-adjustable property of F127 block copolymer PEO99PPO69PEO99, PEO and PPO being poly(ethylene oxide) and poly(propylene oxide), respectively, was found to be useful for the development of automated capillary electrophoresis (CE). The polymer solution can form a gel-like structure with sieving ability and can also serve as a dynamic coating material, thereby effectively suppressing the electroosmotic flow induced by the ionization of the silanol group on the quartz capillary inner wall. When applied to CE as a separation medium, F127 block copolymer can provide the advantages of high separation resolution, easy injection and replacement of the triblock copolymer solution and convenient capillary column treatment. High reproducibility of DNA electrophoretic migration time in CE by replenishing F127 solution in acid-washed capillary tubings can be achieved. The relative standard deviation of the DNA migration time is less than 2%. In the investigation of F127 concentration and temperature effects on the performance of DNA separation in CE, we have found that the DNA electrophoretic migration behavior in the F127 gel-like solution cannot be described by any one of the existing models.
已发现F127嵌段共聚物PEO99PPO69PEO99（其中PEO和PPO分别为聚环氧乙烷和聚环氧丙烷）的粘度可调节特性对自动毛细管电泳（CE）的发展很有用。该聚合物溶液可形成具有筛分能力的凝胶状结构，还可作为动态涂层材料，从而有效抑制石英毛细管内壁上硅醇基电离引起的电渗流。当作为分离介质应用于CE时，F127嵌段共聚物可提供高分离分辨率、三嵌段共聚物溶液易于进样和更换以及毛细管柱处理方便等优点。通过在酸洗毛细管中补充F127溶液，可实现CE中DNA电泳迁移时间的高重现性。DNA迁移时间的相对标准偏差小于2%。在研究F127浓度和温度对CE中DNA分离性能的影响时，我们发现F127凝胶状溶液中DNA的电泳迁移行为无法用任何一种现有模型来描述。
