Department of Neurology, Xiangya Hospital, Central South University, Changsha, China.
Acta Neurol Belg. 2009 Dec;109(4):298-304.
The aim of this study was to determine the correlations among hippocampal damage, spontaneous recurrent seizures (SRS), and mossy fiber sprouting (MFS) using pentylenetetrazole (PTZ) kindling model.
Chronic epileptic model was established by administration of PTZ. Behaviour and EEG seizure activity were recorded. Rats' hippocampus were analyzed with haematoxylin and eosin (H&E) stain for histological lesions and evaluated for MFS with Timm stain.
Prominent MFS was observed in area CA3 rather than the inner molecular layer in PTZ treated rats and the degree of MFS progressed with the development of behavioral kindled seizures. MFS preceded the occurrence of spontaneous seizures. No obvious neuronal necrosis and loss were observed in different regions of the hippocampus during kindling progression.
MFS is not the outcome of SRS. Severe hippocampal damage is not required in the development of MFS and SRS.
本研究旨在使用戊四氮（PTZ）点燃模型确定海马损伤、自发性反复性癫痫发作（SRS）和苔藓纤维发芽（MFS）之间的相关性。
通过给予 PTZ 建立慢性癫痫模型。记录行为和 EEG 癫痫发作活动。对大鼠海马组织进行苏木精和伊红（H&E）染色以进行组织学损伤分析，并使用 Timm 染色评估 MFS。
在 PTZ 处理的大鼠中，MFS 主要出现在 CA3 区而非内分子层，且 MFS 的程度随着行为性点燃性癫痫发作的发展而进展。MFS 先于自发性癫痫发作的发生。在点燃进展过程中，海马的不同区域未观察到明显的神经元坏死和丢失。
MFS 不是 SRS 的结果。在 MFS 和 SRS 的发展过程中，并不需要严重的海马损伤。
