Abdel Azeem Amira A, Mahmoud Amany A, Salaheldine Mostafa M, Amr Khalda
Research Institute of Ophthalmology, National Research Center, Cairo, Egypt.
Bratisl Lek Listy. 2009;110(11):678-83.
To determine the effect of glutathione S-transferases M1 and T1 polymorphisms on the risk of senile cataract among Egyptians.
The glutathione S-transferases (GSTs) are polymorphic enzymes that are important in the protection against oxidative damage.
Using a multiplex polymerase chain reaction (PCR), GSTM1 and GSTT1 gene polymorphisms were evaluated in 53 Egyptians with senile cataract and in 73 healthy individuals of the control group.
The frequency of GSTM1-positive individuals among the senile cataract group was significantly higher than in controls. The risk among the GSTM1-positive individuals of developing senile cataract was even higher in females. It is also increased with combination of "GSTM1-positive and GSTT1-positive" genotypes. However the combination of "GSTM1-null, GSTT1-positive" was found to be protective (OR = 0.47; 95 % CI: 0.22-0.99; p = 0.045).
The GSTMI-positive genotype and the combined "GSTM1-positive/GSTT1-positive" genotype may be associated with an increased risk of development of senile cataract among Egyptians. However, the "GSTM1-null/GSTT1-positive" genotype was found to be protective. Therefore, when evaluating the role of a particular GST gene in disease susceptibility, the whole pattern of different biotransformation enzymes should be taken into account (Tab. 4, Fig. 1, Ref. 36). Full Text (Free, PDF) www.bmj.sk.
确定谷胱甘肽S-转移酶M1和T1基因多态性对埃及人患老年性白内障风险的影响。
谷胱甘肽S-转移酶（GSTs）是多态性酶，在抵御氧化损伤中起重要作用。
采用多重聚合酶链反应（PCR），对53例患老年性白内障的埃及人和73例健康对照组个体的GSTM1和GSTT1基因多态性进行评估。
老年性白内障组中GSTM1阳性个体的频率显著高于对照组。GSTM1阳性个体患老年性白内障的风险在女性中更高。“GSTM1阳性和GSTT1阳性”基因型组合时风险也增加。然而，发现“GSTM1缺失、GSTT1阳性”组合具有保护作用（OR = 0.47；95% CI：0.22 - 0.99；p = 0.045）。
GSTMI阳性基因型以及“GSTM1阳性/GSTT1阳性”组合基因型可能与埃及人患老年性白内障的风险增加有关。然而，发现“GSTM1缺失/GSTT1阳性”基因型具有保护作用。因此，在评估特定GST基因在疾病易感性中的作用时，应考虑不同生物转化酶的整体模式（表4，图1，参考文献36）。全文（免费，PDF）www.bmj.sk 。
