Institute of Analytical Chemistry and Food Chemistry, Graz University of Technology, Graz, Austria.
Anal Chem. 2010 Mar 1;82(5):2124-8. doi: 10.1021/ac902393u.
Magnetic sensor macrospheres (MagSeMacs), i.e., stainless steel spheres coated with optical chemical sensors, are presented as an alternative to existing optical sensor patches and fiber-optical dip-probes. Such spheres can either be reversibly attached to the tip of an optical fiber (dip-probe) or trapped inside a vessel for read-out through the side wall. Moving the magnetic separator at the exterior enables measurements at varying positions with a single sensor. Moreover, the sensor's replacement is rapid and contactless. We measured dissolved oxygen or pH in stirred liquids, rotating flasks, and 24-well plates with a SensorDish-reader device for parallel cell culture monitoring. In these applications, MagSeMacs proved to be advantageous over conventional sensor patches and magnetic optical sensor particles because of their magnetism, spherical shape, reflectance, and size. These properties resulted in strong but reversible fixation, magnetic remote-controllability, short response times, high signal intensities, and simplified handling.
磁性传感器微球（MagSeMacs），即涂有光学化学传感器的不锈钢球体，被提出作为现有光学传感器贴片和光纤浸入式探头的替代品。这些球体可以可逆地附着在光纤（浸入式探头）的尖端，也可以被困在容器内部，通过侧壁进行读取。通过外部移动磁分离器，可以使用单个传感器在不同位置进行测量。此外，传感器的更换快速且非接触式。我们使用 SensorDish-reader 设备在搅拌液体、旋转瓶和 24 孔板中测量溶解氧或 pH 值，用于平行细胞培养监测。在这些应用中，MagSeMacs 由于其磁性、球形、反射率和尺寸，优于传统的传感器贴片和磁性光学传感器颗粒。这些特性导致了强烈但可逆的固定、磁性远程可控性、短的响应时间、高的信号强度和简化的处理。
