Lambertsen Lotte, Brendstrup Mette, Friis Helene, Christensen Jens Jørgen
The Neisseria and Streptococcus Reference Centre, Department for Bacteriology, Mycology and Parasitology, Statens Serum Institut, Copenhagen, Denmark.
Scand J Infect Dis. 2010 May;42(5):333-40. doi: 10.3109/00365540903501616.
The aim of this study was to characterize invasive penicillin non-susceptible pneumococci (PNSP) isolated in Denmark from 2001 to 2005, describe the genetic changes in the penicillin-binding proteins (PBPs) and compare the isolates to the international recognized PNSP clones. All isolates were characterized using serotyping; 43 isolates were also characterized by multilocus sequence typing (MLST) and sequence determination of the PBP genes, pbp1a, pbp2b and pbp2x. In addition, isolates were tested for susceptibility to various antimicrobial agents. From 2001 to 2005, 178 invasive PNSP isolates were found among all invasive isolates. Of these, 13% were penicillin-resistant pneumococci (PRP). The PRP isolates were different to the penicillin intermediate-resistant pneumococci (PIRP) as they were co-resistant to a higher number of antimicrobial agents, and the serotype diversity was lower for the PRP isolates compared to the PIRP isolates. Changes in the PBPs were found to be the same as those observed in other studies. Most PRP isolates were found to belong to the clonal complex CC156 of which most were ST156 serotype 9V or 14. ST156 is a well known multiresistant widespread international PMEN (Pneumococcal Molecular Epidemiology Network) clone, Spain(9V)-3, which is also present as one of the dominant clones in our neighbour countries of Sweden, Norway and Germany.
本研究的目的是对2001年至2005年在丹麦分离出的侵袭性青霉素不敏感肺炎球菌（PNSP）进行特征分析，描述青霉素结合蛋白（PBPs）的基因变化，并将这些分离株与国际公认的PNSP克隆进行比较。所有分离株均采用血清分型进行特征分析；43株分离株还通过多位点序列分型（MLST）以及PBP基因pbp1a、pbp2b和pbp2x的序列测定进行特征分析。此外，还对分离株进行了多种抗菌药物的敏感性测试。在2001年至2005年期间，在所有侵袭性分离株中发现了178株侵袭性PNSP分离株。其中，13%为青霉素耐药肺炎球菌（PRP）。PRP分离株与青霉素中介耐药肺炎球菌（PIRP）不同，因为它们对更多种类的抗菌药物具有共同耐药性，并且与PIRP分离株相比，PRP分离株的血清型多样性较低。PBPs的变化与其他研究中观察到的变化相同。大多数PRP分离株属于克隆复合体CC156，其中大多数是ST156血清型9V或14。ST156是一种众所周知的多耐药广泛传播的国际肺炎球菌分子流行病学网络（PMEN）克隆，即西班牙（9V）-3，在我们的邻国瑞典、挪威和德国也是主要克隆之一。
