Université Lyon 1, UFR Lyon Sud, Pierre-Bénite, France.
Allergy. 2010 Aug;65(8):996-1003. doi: 10.1111/j.1398-9995.2009.02307.x. Epub 2010 Feb 1.
Delayed allergic skin reactions to drugs are common iatrogenic diseases mediated by activation of specific T cells in the skin.
To better understand the role of T cells in these diseases, we developed a mouse model of drug allergy induced by skin sensitization to amoxicillin (amox), a penicillin antibiotic frequently involved in delayed drug allergy.
Whereas wild-type mice could not be sensitized to amox, CD4+ T-cell-deficient mice developed an amox-specific allergic skin response, mediated by IFN-gamma-producing CD8+ T cells. Amox-specific CD8+ T cells, induced in lymphoid organs at a high frequency during sensitization, were recruited in the skin upon challenge. CD8+ T cells were effectors of the allergic skin reaction to amox as in vivo treatment with depleting anti-CD8 mAbs abrogated the skin inflammatory reaction and as purified CD8+ T cells could adoptively transfer the allergic response to naive recipients.
CD8+ T cells mediate penicillin skin allergy.
药物迟发性过敏皮肤反应是由皮肤中特定 T 细胞激活引起的常见医源性疾病。
为了更好地了解 T 细胞在这些疾病中的作用，我们建立了一种通过对青霉素类抗生素阿莫西林（amox）致敏诱导的药物过敏的小鼠模型。
虽然野生型小鼠不能对 amox 致敏，但 CD4+T 细胞缺陷型小鼠会产生由 IFN-γ产生的 CD8+T 细胞介导的 amox 特异性过敏皮肤反应。在致敏过程中，淋巴器官中高频率诱导的 amox 特异性 CD8+T 细胞在受到挑战时会募集到皮肤中。CD8+T 细胞是对 amox 过敏皮肤反应的效应物，因为体内用耗竭性抗 CD8 mAb 治疗可消除皮肤炎症反应，并且纯化的 CD8+T 细胞可以将过敏反应转移给未致敏的受体。
CD8+T 细胞介导青霉素皮肤过敏。
