Department of Otolaryngology, Clinical Microbiology Laboratory, Ataturk Training and Research Hospital, Izmir, Turkey.
J Otolaryngol Head Neck Surg. 2010 Feb;39(1):52-5.
We investigated the presence of biofilms in patients with chronic rhinosinusitis.
Prospective study.
Thirty patients with chronic rhinosinusitis scheduled for endoscopic sinus surgery were enrolled in this study. Nasal swab cultures were taken at the time of surgery. Samples were cultured using conventional methods for Haemophilus influenzae, Pseudomonas aeruginosa, Streptococcus pneumoniae, and Staphylococcus aureus.
Culture-positive samples were evaluated for slime-forming ability. Qualitative detection of biofilm formation by all slime-producing strains was studied by culturing the strains on Congo red agar plates.
Twenty-three of 30 patients' samples were culture positive (76.6%). Thirteen S. aureus and 10 P. aeruginosa cultures were identified on 23 specimens. Bacterial biofilms were present on 15 of 23 (65.2%) culture-positive specimens. Nine of 13 (69.2%) S. aureus cultures and 6 of 10 (60%) P. aeruginosa cultures produced bacterial biofilms.
This conventional method is capable of demonstrating biofilm-forming ability in bacteria recovered from patients with chronic rhinosinusitis. The biofilm-forming ability was higher in S. aureus compared with P. aeruginosa.
研究慢性鼻-鼻窦炎患者生物膜的存在情况。
前瞻性研究。
本研究纳入了 30 例拟行内镜鼻窦手术的慢性鼻-鼻窦炎患者。手术时采集鼻拭子培养。采用常规方法培养流感嗜血杆菌、铜绿假单胞菌、肺炎链球菌和金黄色葡萄球菌。
培养阳性的样本评估其产粘液能力。通过在刚果红琼脂平板上培养所有产粘液的菌株，定性检测生物膜的形成。
30 例患者中有 23 例（76.6%）样本培养阳性。23 个标本中鉴定出 13 株金黄色葡萄球菌和 10 株铜绿假单胞菌。23 个培养阳性标本中有 15 个（65.2%）存在细菌生物膜。13 株金黄色葡萄球菌中有 9 株（69.2%）和 10 株铜绿假单胞菌中有 6 株（60%）产生细菌生物膜。
本常规方法能够证明从慢性鼻-鼻窦炎患者中分离出的细菌具有形成生物膜的能力。与铜绿假单胞菌相比，金黄色葡萄球菌形成生物膜的能力更高。
