Department of Surgery-Otorhinolaryngology, Head and Neck Surgery, University of Adelaide, Adelaide, Australia.
Am J Rhinol Allergy. 2009 Nov-Dec;23(6):556-61. doi: 10.2500/ajra.2009.23.3413.
Conclusive evidence exists that biofilms are present on the mucosa of chronic rhinosinusitis (CRS) patients. Less is known about the species constituting these biofilms. This study developed a fluorescence in situ hybridization (FISH) protocol for characterization of bacterial and fungal biofilms in CRS.
Fifty CRS patients and 10 controls were recruited. Bacteria FISH probes for Staphylococcus aureus, Haemophilus influenzae, and Pseudomonas aeruginosa and a universal probe for fungi were applied to sinus mucosal specimens and then analyzed using confocal scanning laser microscopy.
Thirty-six of 50 CRS patients had biofilms present in contrast to 0/10 controls, suggesting a role for biofilms in the pathogenesis of this disease. S. aureus was the most common biofilm-forming organism. Eleven of 50 CRS patients had characteristic fungal biofilms present.
This is the largest study of biofilms in CRS. It has validated mucosal tissue cryopreservation for delayed biofilm analysis. Fungal biofilms have been identified and the importance of S. aureus biofilms in the polymicrobial etiology of CRS is highlighted.
有确凿的证据表明，慢性鼻-鼻窦炎（CRS）患者的黏膜上存在生物膜。关于构成这些生物膜的物种知之甚少。本研究开发了一种荧光原位杂交（FISH）方案，用于表征 CRS 中的细菌和真菌生物膜。
招募了 50 名 CRS 患者和 10 名对照者。将用于金黄色葡萄球菌、流感嗜血杆菌和铜绿假单胞菌的细菌 FISH 探针以及用于真菌的通用探针应用于鼻窦黏膜标本，然后使用共聚焦扫描激光显微镜进行分析。
与 0/10 名对照者相比，50 名 CRS 患者中有 36 名存在生物膜，这表明生物膜在该疾病的发病机制中起作用。金黄色葡萄球菌是最常见的生物膜形成菌。11 名 CRS 患者存在特征性的真菌生物膜。
这是 CRS 生物膜的最大研究。它验证了用于延迟生物膜分析的黏膜组织冷冻保存。已鉴定出真菌生物膜，并强调了金黄色葡萄球菌生物膜在 CRS 多微生物病因学中的重要性。
