Department of Forensic Medicine, Shantou University Medical College, Shantou 515041, Guangdong Province, PR China.
J Trace Elem Med Biol. 2010 Jan;24(1):36-41. doi: 10.1016/j.jtemb.2009.08.002. Epub 2009 Sep 26.
Patients with a severe trauma exhibit a strong oxidative stress, an intense inflammatory response, and long-lasting hypermetabolism, all of which are proportional to the severity of injury. In this study, we investigated the impact of trace element (TE) supplementation on the inflammatory response in an animal model of major trauma. New Zealand White rabbits were randomly assigned as a control group (n=5) and an experimental group (n=70) that, after receiving a major trauma, was subdivided into Trauma-Control (n=35) and Trauma-TE (n=35) groups. Systemic inflammatory response syndrome (SIRS) was observed in 40 out of 70 rabbits with a trauma, with a higher incidence in the Trauma-Control group (88.6%; 31/35) than the Trauma-TE group (28.6%; 10/35) (p<0.01). The mortality rate was significantly different between the Trauma-Control and the Trauma-TE groups; (34% vs. 8%; p<0.01). There were significant post-trauma alterations in the levels of (1) serum and spleen zinc (Zn), copper (Cu), selenium (Se), and manganese (Mn), (2) serum AST and ALT, (3) serum interleukin-6/10, and (4) nuclear factor kappa binding (NF-kappaB) activity and the expression. TE supplementation: (1) improved blood urea nitrogen (BUN), and creatinine (Cr) levels, (2) stabilized IL-6/10 production, (3) decreased NF-kappaB p(65) production. Appropriate TE supplementation can improve the TE status, mitigate SIRS, and reduce the mortality due to multiple organ dysfunction syndromes (MODS)/multiple organ failure (MOF) after major trauma.
创伤患者表现出强烈的氧化应激、强烈的炎症反应和持久的高代谢，所有这些都与损伤的严重程度成正比。在这项研究中，我们研究了微量元素 (TE) 补充对大型创伤动物模型中炎症反应的影响。新西兰白兔被随机分为对照组 (n=5) 和实验组 (n=70)，实验组在接受重大创伤后又分为创伤对照组 (n=35) 和创伤 TE 组 (n=35)。70 只兔子中有 40 只出现全身性炎症反应综合征 (SIRS)，创伤对照组的发病率较高 (88.6%；31/35) ，而创伤 TE 组的发病率较低 (28.6%；10/35) (p<0.01)。创伤对照组和创伤 TE 组的死亡率差异显著；(34%比 8%；p<0.01)。创伤后血清和脾脏锌 (Zn)、铜 (Cu)、硒 (Se) 和锰 (Mn) 水平、(2) 血清 AST 和 ALT、(3) 血清白细胞介素-6/10 和 (4) 核因子 kappa 结合 (NF-kappaB) 活性和表达均有明显改变。TE 补充：(1) 改善血尿素氮 (BUN) 和肌酐 (Cr) 水平，(2) 稳定 IL-6/10 产生，(3) 减少 NF-kappaB p(65) 产生。适当的 TE 补充可以改善 TE 状态，减轻 SIRS，并降低因多器官功能障碍综合征 (MODS)/多器官衰竭 (MOF) 而导致的死亡率。
