Department of Surgery, Brigham and Women's Hospital and Harvard Medical School Boston, MA 02115, USA.
Shock. 2012 Aug;38(3):314-9. doi: 10.1097/SHK.0b013e3182627338.
Zinc ions (Zn) are essential for tissue repair following injury or stress. We hypothesize that during such stresses Zn is redistributed to labile pools in plasma components. Here we tested this hypothesis using a novel assay to monitor labile Zn in plasma in hemorrhagic shock. Adult rats in the shock group (S group) underwent hemorrhage and resuscitation. Blood samples were drawn at baseline and at 1, 4, and 24 h. The surgical control group (SC group) was anesthetized and instrumented, but not bled. Albumin, total Zn, and labile Zn levels were assayed in plasma. Binding capacity for Zn was assessed in high- and low-molecular-weight pools. Significant decreases in total Zn were observed by 24 h, in both S and SC groups. Albumin levels were significantly reduced in the S group at 1 and 4 h but restored at 24 h; significant changes were not observed in other groups. In whole plasma, labile Zn levels were stable initially in the S and SC groups, but declined at 24 h. In the high-molecular-weight pool, marked and significant impairment of binding was noted throughout all time periods following the shock period in the S group. Such changes were observed in the SC group of less intensity and duration. These experiments suggest that shock alters affinity of plasma proteins for Zn, promoting delivery to peripheral tissues during periods of increased Zn utilization.
锌离子（Zn）是组织损伤或应激后修复所必需的。我们假设，在这种应激下，Zn 会重新分配到血浆成分中的不稳定池中。在这里，我们使用一种新的测定法来测试这种假说，以监测失血性休克患者血浆中的可利用锌。休克组（S 组）中的成年大鼠接受了出血和复苏。在基线和 1、4 和 24 小时时抽取血样。手术对照组（SC 组）接受了麻醉和仪器操作，但未出血。测定血浆中的白蛋白、总 Zn 和可利用 Zn 水平。评估 Zn 的结合能力在高分子和低分子池中。在 S 和 SC 组中，24 小时时观察到总 Zn 显著降低。S 组在 1 和 4 小时时白蛋白水平显著降低，但在 24 小时时恢复正常；其他组未观察到显著变化。在全血浆中，S 和 SC 组的可利用 Zn 水平在最初是稳定的，但在 24 小时时下降。在高分子池中，在休克期后的所有时间内，S 组的结合能力明显且显著受损。SC 组的变化强度和持续时间较短。这些实验表明，休克改变了血浆蛋白对 Zn 的亲和力，在 Zn 利用率增加的时期促进了向周围组织的输送。
