用于 PET 探针的纯化和代谢产物分析的超快速 LC 方法。
Ultrafast LC method for purification and metabolite analysis of PET probes.
机构信息
Molecular Imaging Center, National Institute of Radiological Sciences, Chiba, Japan.
出版信息
Nucl Med Biol. 2010 Jan;37(1):67-72. doi: 10.1016/j.nucmedbio.2009.08.002. Epub 2009 Oct 3.
An ultrafast and efficient high-performance liquid chromatographic (LC) method was developed to purify positron emission tomography (PET) radiopharmaceuticals as well as for metabolite analysis of the plasma sample. Chromatographic separation was achieved on a short (60 mm length) semipreparative (10 mm I.D.) column packed with 2.5-mum particles using a mixture of acetonitrile and sodium phosphate buffer as the mobile phase at a flow rate of 8-10 ml/min. Under the optimum conditions, excellent separation of the target PET probe was obtained from chemical/radiochemical impurities or radioactive metabolites with a very short run time of 2 min. This characteristic enabled significant shortening of the purification and evaporation processes in the production of short-lived radiopharmaceuticals and highly sensitive radiometric analysis with good temporal resolution during the metabolism study.
建立了一种超快速、高效的液相色谱（LC）方法，用于纯化正电子发射断层扫描（PET）放射性药物，以及分析血浆样品中的代谢物。在最佳条件下，使用 2.5-μm 颗粒填充的短（60mm 长）半制备（10mm ID）柱，以乙腈和磷酸钠缓冲液的混合物作为流动相，流速为 8-10ml/min，可实现目标 PET 探针与化学/放射性化学杂质或放射性代谢物的出色分离，运行时间仅为 2 分钟。这一特点使得在生产短寿命放射性药物时，显著缩短了纯化和蒸发过程，并在代谢研究中实现了高灵敏度的放射性分析和良好的时间分辨率。
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