Molecular Imaging Center, National Institute of Radiological Sciences, Chiba, Japan.
Nucl Med Biol. 2010 Jan;37(1):67-72. doi: 10.1016/j.nucmedbio.2009.08.002. Epub 2009 Oct 3.
An ultrafast and efficient high-performance liquid chromatographic (LC) method was developed to purify positron emission tomography (PET) radiopharmaceuticals as well as for metabolite analysis of the plasma sample. Chromatographic separation was achieved on a short (60 mm length) semipreparative (10 mm I.D.) column packed with 2.5-mum particles using a mixture of acetonitrile and sodium phosphate buffer as the mobile phase at a flow rate of 8-10 ml/min. Under the optimum conditions, excellent separation of the target PET probe was obtained from chemical/radiochemical impurities or radioactive metabolites with a very short run time of 2 min. This characteristic enabled significant shortening of the purification and evaporation processes in the production of short-lived radiopharmaceuticals and highly sensitive radiometric analysis with good temporal resolution during the metabolism study.
建立了一种超快速、高效的液相色谱（LC）方法，用于纯化正电子发射断层扫描（PET）放射性药物，以及分析血浆样品中的代谢物。在最佳条件下，使用 2.5-μm 颗粒填充的短（60mm 长）半制备（10mm ID）柱，以乙腈和磷酸钠缓冲液的混合物作为流动相，流速为 8-10ml/min，可实现目标 PET 探针与化学/放射性化学杂质或放射性代谢物的出色分离，运行时间仅为 2 分钟。这一特点使得在生产短寿命放射性药物时，显著缩短了纯化和蒸发过程，并在代谢研究中实现了高灵敏度的放射性分析和良好的时间分辨率。
