Karolinska Institutet, Department of Clinical Neuroscience, Center for Psychiatric Research, Stockholm SE-171 76, Sweden.
J Chromatogr B Analyt Technol Biomed Life Sci. 2012 May 1;895-896:116-22. doi: 10.1016/j.jchromb.2012.03.028. Epub 2012 Mar 28.
A fast and sensitive liquid chromatographic (fast-LC) method with radiometric detection was developed and validated to analyze positron emission tomography (PET) radioligands in plasma during PET studies. The plasma samples were deproteinized with acetonitrile and the extracts were injected into the fast-LC system coupled to an on-line radioactivity detector. Under the optimum conditions, complete separation of target PET radioligands from their radioactive metabolites was achieved within the short run time of only 3.5-min. The limits of detection were 1.0-1.2 Becquerel (Bq) for (11)C and (18)F-labeled compounds. This method can successfully be applied to study the metabolism of a wide variety of PET radioligands in human and monkey plasma with higher numbers of samples to be analyzed compared to the traditional LC method.
建立并验证了一种快速灵敏的液相色谱（fast-LC）放射性检测法，用于分析正电子发射断层扫描（PET）研究中血浆中的 PET 放射性配体。用乙腈使血浆样品去蛋白，提取液注入 fast-LC 系统，与在线放射性检测器相连。在最佳条件下，目标 PET 放射性配体与其放射性代谢物在仅 3.5 分钟的短运行时间内完全分离。（11）C 和（18）F 标记化合物的检测限为 1.0-1.2 贝克勒尔（Bq）。与传统的 LC 方法相比，该方法可成功应用于分析更多的人及猴血浆中各种 PET 放射性配体的代谢。
