阿根廷恰加斯病流行地区的克氏锥虫特异性免疫应答。
Trypanosoma cruzi-specific immune responses in subjects from endemic areas of Chagas disease of Argentina.
机构信息
Instituto Nacional de Parasitología Dr. Mario Fatala Chaben, Av. Paseo Colón 568, Buenos Aires 1063, Argentina.
出版信息
Microbes Infect. 2010 May;12(5):359-63. doi: 10.1016/j.micinf.2010.01.011. Epub 2010 Feb 1.
Abstract
Trypanosoma cruzi-specific immune responses were evaluated in a total of 88 subjects living in areas endemic of Chagas disease of Argentina by IFN-gamma ELISPOT assays and immunoblotting. Positive T. cruzi antigen-induced IFN-gamma responses were detected in 42% of subjects evaluated (15/26 positive by conventional serology and 22/62 seronegative subjects). Using immunoblotting, T. cruzi-specific IgG reactivity was detected in all seropositive subjects and in 11% (7/61) of subjects negative by conventional serology. Measurements of T cell responses and antibodies by immunoblotting, in conjunction with conventional serology, might enhance the capability of detection of exposure to T. cruzi in endemic areas.
摘要
采用 IFN-γ ELISPOT assays 和免疫印迹法，评估了居住在阿根廷恰加斯病流行地区的 88 名受试者的克氏锥虫特异性免疫应答。在评估的受试者中，有 42%（15/26 例常规血清学阳性和 22/62 例血清学阴性的受试者）检测到克氏锥虫抗原诱导的 IFN-γ 阳性反应。通过免疫印迹法，在所有血清学阳性的受试者和 11%（7/61）的常规血清学阴性的受试者中检测到克氏锥虫特异性 IgG 反应性。结合常规血清学检测，通过免疫印迹法测量 T 细胞应答和抗体，可能会提高在流行地区检测接触克氏锥虫的能力。
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