Nhp6：酿酒酵母中一种虽小但功能强大的染色质结构效应因子。
Nhp6: a small but powerful effector of chromatin structure in Saccharomyces cerevisiae.
作者信息
Stillman David J
机构信息
Department of Pathology, University of Utah Health Sciences Center, Salt Lake City, UT 84112, USA.
出版信息
Biochim Biophys Acta. 2010 Jan-Feb;1799(1-2):175-80. doi: 10.1016/j.bbagrm.2009.11.010.
Abstract
The small Nhp6 protein from budding yeast is an abundant protein that binds DNA non-specifically and bends DNA sharply. It contains only a single HMGB domain that binds DNA in the minor groove and a basic N-terminal extension that wraps around DNA to contact the major groove. This review describes the genetic and biochemical experiments that indicate Nhp6 functions in promoting RNA pol III transcription, in formation of preinitiation complexes at promoters transcribed by RNA pol II, and in facilitating the activity of chromatin modifying complexes. The FACT complex may provide a paradigm for how Nhp6 functions with chromatin factors, as Nhp6 allows Spt16-Pob3 to bind to and reorganize nucleosomes in vitro.
摘要
来自芽殖酵母的小Nhp6蛋白是一种丰富的蛋白质，它能非特异性地结合DNA并使DNA急剧弯曲。它仅包含一个在小沟中结合DNA的单一HMGB结构域和一个环绕DNA以接触大沟的碱性N端延伸部分。这篇综述描述了遗传和生化实验，这些实验表明Nhp6在促进RNA聚合酶III转录、在RNA聚合酶II转录的启动子处形成起始前复合物以及在促进染色质修饰复合物的活性方面发挥作用。FACT复合物可能为Nhp6如何与染色质因子协同作用提供了一个范例，因为Nhp6能使Spt16 - Pob3在体外结合并重组核小体。
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本文引用的文献
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The E2F functional analogue SBF recruits the Rpd3(L) HDAC, via Whi5 and Stb1, and the FACT chromatin reorganizer, to yeast G1 cyclin promoters.E2F功能类似物SBF通过Whi5和Stb1，将Rpd3(L)组蛋白去乙酰化酶以及FACT染色质重组因子招募至酵母G1期细胞周期蛋白启动子。
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