Varner D D, Vaughan S D, Johnson L
Department of Large Animal Medicine, College of Veterinary Medicine, Texas A&M University, College Station 77843-4475.
Am J Vet Res. 1991 Feb;52(2):224-30.
Three ejaculates from each of 3 stallions were used to evaluate a computerized system (Hamilton-Thorn motility analyzer; HTMA) for measuring equine spermatozoal motility. Variance components (ejaculate-within-stallion, chamber-within-ejaculate, and microscopic field-within-chamber) were determined for each stallion after diluting ejaculates to 25 x 10(6) spermatozoa/ml with a skim milk-glucose seminal extender. The HTMA was compared with frame-by-frame playback videomicrography (VIDEO) for determining: percentage of spermatozoal motility and spermatozoal number in microscopic fields; curvilinear velocity and straight-line velocity of individual spermatozoa for 5 track types; and repeatability of those velocity measurements. The effect of spermatozoal number per microscopic field on incidence of intersecting spermatozoa and the outcome of intersecting spermatozoa also were evaluated. Greatest variability in motility measures was generally attributed to the microscopic field-within-chamber component. The HTMA was highly correlated with VIDEO for estimation of spermatozoal numbers per microscopic field (r = 0.99; P less than 0.001) and motility (r = 0.97; P less than 0.001); however over the entire range of spermatozoal numbers, the HTMA yielded higher spermatozoal numbers per microscopic field (P less than 0.05) and higher motility (P less than 0.05) than did VIDEO. The HTMA- and VIDEO-derived measurements of curvilinear and straight-line velocities were highly correlated for all spermatozoal track types, but both measures were higher (P less than 0.05) by use of the HTMA than by use of VIDEO for most track types. For 3 of 5 track types, measurements of curvilinear and straight-line velocities were less variable (P less than 0.05), using the HTMA, rather than VIDEO.(ABSTRACT TRUNCATED AT 250 WORDS)
使用3匹种马的每份3次射精来评估一种用于测量马精子活力的计算机系统（汉密尔顿-桑恩活力分析仪；HTMA）。用脱脂奶-葡萄糖精液稀释剂将射精精液稀释至25×10⁶精子/毫升后，确定每匹种马的方差成分（种马内射精、射精内腔室、腔室内显微镜视野）。将HTMA与逐帧回放视频显微镜检查（VIDEO）进行比较，以确定：显微镜视野中精子活力百分比和精子数量；5种轨迹类型的单个精子的曲线速度和直线速度；以及这些速度测量的重复性。还评估了每个显微镜视野中精子数量对交叉精子发生率和交叉精子结果的影响。活力测量中最大的变异性通常归因于腔室内显微镜视野成分。HTMA与VIDEO在估计每个显微镜视野中的精子数量（r = 0.99；P＜0.001）和活力（r = 0.97；P＜0.001）方面高度相关；然而，在整个精子数量范围内，HTMA在每个显微镜视野中产生的精子数量更高（P＜0.05），活力也更高（P＜0.05）。对于所有精子轨迹类型，HTMA和VIDEO得出的曲线和直线速度测量值高度相关，但对于大多数轨迹类型，使用HTMA时这两种测量值均高于使用VIDEO时（P＜0.05）。对于5种轨迹类型中的3种，使用HTMA而非VIDEO时，曲线和直线速度的测量值变异性较小（P＜0.05）。（摘要截短于250字）
