Adair H S, Goble D O, Vanhooser S, Blackford J T, Rohrbach B W
Department of Rural Practice, College of Veterinary Medicine, University of Tennessee, Knoxville 37901-1071.
Am J Vet Res. 1991 Feb;52(2):333-6.
The antebrachiocarpal and tarsocrural joints of 10 adult horses were randomly assigned to 1 of 4 groups. Groups were formulated and were treated as follows: group 1, control (arthrocentesis only); group 2, buffered lactated Ringer solution; group 3, 10% dimethyl sulfoxide (DMSO; w/v) in lactated Ringer solution; and group 4, 30% DMSO (w/v) in lactated Ringer solution. Joints were lavaged once with the respective solution. Prior to lavage and on days 1, 4, and 8 after lavage, all horses were evaluated for lameness and joint effusion; synovial fluid total and differential WBC counts, synovial fluid total protein concentration, and mucin clot quality were determined. Horses were euthanatized on day 8, and joints were evaluated grossly, histologically, and histochemically. Significant difference was not observed in effect of lactated Ringer solution, 10% DMSO, and 30% DMSO on any measured variable. At 24 hours after treatment, significant (P less than 0.05) difference in synovial fluid WBC numbers and total protein concentration was detected between control and treated joints. Eighty percent of lavaged joints had effusion 24 hours after treatment, compared with 30% of control joints. Gross, histopathologic, or histochemical differences were not detected between treated and control joints. Results of the study indicate that buffered lactated Ringer, 10% DMSO, and 30% DMSO solutions induce similar inflammatory changes in articular structures and significantly greater inflammatory reaction than does arthrocentesis alone.
将10匹成年马的腕掌关节和跗关节随机分为4组中的1组。分组如下并进行相应处理：第1组为对照组（仅进行关节穿刺术）；第2组为乳酸林格氏缓冲液；第3组为乳酸林格氏液中含10%二甲亚砜（DMSO；重量/体积）；第4组为乳酸林格氏液中含30% DMSO（重量/体积）。用相应溶液对关节进行一次灌洗。在灌洗前以及灌洗后第1、4和8天，对所有马匹进行跛行和关节积液评估；测定滑液白细胞总数及分类计数、滑液总蛋白浓度和粘蛋白凝块质量。在第8天对马匹实施安乐死，并对关节进行大体、组织学和组织化学评估。未观察到乳酸林格氏液、10% DMSO和30% DMSO对任何测量变量的影响存在显著差异。在治疗后24小时，对照组和治疗组关节的滑液白细胞数量和总蛋白浓度存在显著（P<0.05）差异。治疗后24小时，80%的灌洗关节出现积液，而对照组关节为30%。治疗组和对照组关节之间未检测到大体、组织病理学或组织化学差异。研究结果表明，乳酸林格氏缓冲液、10% DMSO和30% DMSO溶液在关节结构中引起的炎症变化相似，且比单纯关节穿刺术引起的炎症反应显著更大。
