Sotelo Eric Danilo Pauls, Vendruscolo Cynthia Prado, Fülber Joice, Seidel Sarah Raphaela Torquato, Jaramillo Fernando Mosquera, Agreste Fernanda Rodrigues, Silva Luís Cláudio Lopes Correia da, Baccarin Raquel Yvonne Arantes
Departamento de Clínica Médica, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo 05508-270, Brazil.
Departamento de Cirurgia, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo 05508-270, Brazil.
Vet Sci. 2020 Apr 30;7(2):57. doi: 10.3390/vetsci7020057.
Several studies in human and equine medicine have produced controversial results regarding the role of dimethylsulfoxide (DMSO) as a therapeutic agent. This study aimed to evaluate the effect of joint lavage with different DMSO concentrations on biomarkers of synovial fluid inflammation and cartilage degradation in joints with lipopolysaccharide (LPS)-induced synovitis. Twenty-six tibiotarsal joints of 13 horses were randomly distributed into four groups (lactated Ringer's solution; 5% DMSO in lactated Ringer's; 10% DMSO in lactated Ringer's; and sham). All animals were evaluated for the presence of lameness, and synovial fluid analyses were performed at 0 h, 1 h, 8 h, 24 h, and 48 h (T0, T1, T8, T24, and T48, respectively). The white blood cell counts (WBC), total protein (TP), urea, prostaglandin E2 (PGE2), interleukin (IL)-1β, IL-6, IL-10, tumor necrosis factor-α (TNF-α), hyaluronic acid (HA), and chondroitin sulfate (CS) concentrations were measured. The WBC counts and PGE2, IL-1β, IL-6, and TP concentrations increased in all groups at T8 compared to baseline values (<0.05). At T48, only the 5% DMSO and 10% DMSO groups showed a significant decrease in WBC counts (<0.05). Furthermore, the 10% DMSO group had lower concentrations of PGE2 and IL-1β at T48 than at T8 (<0.05) and presented lower IL-6 levels than the5% DMSO and lactated Ringer's groups at T24. All groups showed an increase in CS concentration after LPS-induced synovitis. Joint lavage with 10% DMSO in lactated Ringer´s has anti-inflammatory but not chondroprotective effects.
在人和马医学领域的多项研究中，关于二甲基亚砜（DMSO）作为一种治疗剂的作用产生了有争议的结果。本研究旨在评估用不同浓度DMSO进行关节灌洗对脂多糖（LPS）诱导的滑膜炎关节中滑液炎症生物标志物和软骨降解的影响。将13匹马的26个跗关节随机分为四组（乳酸林格氏液；乳酸林格氏液中含5%DMSO；乳酸林格氏液中含10%DMSO；假手术组）。对所有动物进行跛行检查，并在0小时、1小时、8小时、24小时和48小时（分别为T0、T1、T8、T24和T48）进行滑液分析。测量白细胞计数（WBC）、总蛋白（TP）、尿素、前列腺素E2（PGE2）、白细胞介素（IL）-1β、IL-6、IL-10、肿瘤坏死因子-α（TNF-α）、透明质酸（HA）和硫酸软骨素（CS）浓度。与基线值相比，所有组在T8时WBC计数以及PGE2、IL-1β、IL-6和TP浓度均升高（<0.05）。在T48时，只有5%DMSO组和10%DMSO组的WBC计数显著降低（<0.05）。此外，10%DMSO组在T48时PGE2和IL-1β的浓度低于T8时（<0.05），并且在T24时IL-6水平低于5%DMSO组和乳酸林格氏液组。LPS诱导滑膜炎后，所有组的CS浓度均升高。在乳酸林格氏液中加入10%DMSO进行关节灌洗具有抗炎作用，但不具有软骨保护作用。
