Department of Internal Medicine, Kaohsiung Medical University Chung-Ho Memorial Hospital, Kaohsiung, Taiwan.
Kaohsiung J Med Sci. 2010 Feb;26(2):68-75. doi: 10.1016/s1607-551x(10)70010-3.
Toona sinensis extracts have been shown to exhibit anti-cancer effects in human ovarian cancer cell lines, human promyelocytic leukemia cells and human lung adenocarcinoma. Its safety has also been confirmed in animal studies. However, its anti-cancer properties in human lung large cell carcinoma have not been studied. Here, we used a powder obtained by freeze-drying the supernatant of centrifuged crude extract from Toona sinensis leaves (TSL-1) to treat the human lung carcinoma cell line H661. Cell viability was evaluated by the 3-(4-,5-dimethylthiazol-2-yl)-2,5 diphenyl tetrazolium bromide assay. Flow cytometry analysis revealed that TSL-1 blocked H661 cell cycle progression. Western blot analysis showed decreased expression of cell cycle proteins that promote cell cycle progression, including cyclin-dependent kinase 4 and cyclin D1, and increased the expression of proteins that inhibit cell cycle progression, including p27. Furthermore, flow cytometry analysis showed that TSL-1 induced H661 cell apoptosis. Western blot analysis showed that TSL-1 reduced the expression of the anti-apoptotic protein B-cell lymphoma 2, and degraded the DNA repair protein, poly(ADP-ribose) polymerase. TSL-1 shows potential as a novel therapeutic agent or for use as an adjuvant for treating human lung large cell carcinoma.
香椿提取物已被证明在人卵巢癌细胞系、人早幼粒细胞白血病细胞和人肺腺癌细胞中具有抗癌作用。其在动物研究中也已被证实安全性良好。然而，其在人肺大细胞癌中的抗癌特性尚未得到研究。在这里，我们使用从香椿叶中离心粗提物上清液冻干得到的粉末（TSL-1）来处理人肺癌细胞系 H661。通过 3-（4,5-二甲基噻唑-2-基）-2,5-二苯基四氮唑溴盐法评估细胞活力。流式细胞术分析显示 TSL-1 阻止了 H661 细胞周期进程。Western blot 分析表明，促进细胞周期进程的细胞周期蛋白依赖性激酶 4 和细胞周期蛋白 D1 表达减少，抑制细胞周期进程的蛋白表达增加，包括 p27。此外，流式细胞术分析显示 TSL-1 诱导了 H661 细胞凋亡。Western blot 分析表明，TSL-1 降低了抗凋亡蛋白 B 细胞淋巴瘤 2 的表达，并降解了 DNA 修复蛋白聚（ADP-核糖）聚合酶。TSL-1 有望成为治疗人肺大细胞癌的新型治疗剂或辅助剂。
