没食子酸（香椿叶提取物的主要成分）对口腔鳞状癌细胞的抗肿瘤作用。

Anti-neoplastic effects of gallic acid, a major component of Toona sinensis leaf extract, on oral squamous carcinoma cells.

机构信息

Department of Food Science and Technology, Ta-Jen University, Ping Tung Hsien, Taiwan.

出版信息

Molecules. 2010 Nov 16;15(11):8377-89. doi: 10.3390/molecules15118377.

DOI:10.3390/molecules15118377

PMID:21081858

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6259246/

Abstract

Extract of Toona sinensis (TS) has been reported to have various effects on cultured cell lines, including anti-proliferative activity in cancer cells. We have studied the effects of TS on various human oral squamous carcinoma cell lines (HOSCC), including UM1, UM2, SCC-4, and SCC-9. These cell lines were treated with TS leaf extract and screened for viability, apoptosis, necrosis, and apoptotic gene expression. Normal human oral keratinocytes (NHOK) served as a control for cytotoxic assays. Viability of TS-treated HOSCC was reduced, whereas that of NHOK was not affected. FACScan analysis revealed that the leaf extract induced apoptosis or a combination of apoptosis and necrosis, depending on cell type. Microarray and semi-quantitative RT-PCR analysis for apoptotic-related gene expression revealed that 3,4,5-trihydroxybenzoic acid (gallic acid, one of the major bioactive compounds purified from TS extract) up-regulated pro-apoptotic genes such TNF-α, TP53BP2, and GADD45A, and down-regulated the anti-apoptotic genes Survivin and cIAP1, resulting in cell death. This study suggests that gallic acid, the major bioactive compound present, is responsible for the anti-neoplastic effect of Toona sinensis leaf extract.

摘要

香椿提取物（TS）已被报道对培养的细胞系具有多种作用，包括在癌细胞中的抗增殖活性。我们研究了 TS 对各种人口腔鳞状细胞癌细胞系（HOSCC）的影响，包括 UM1、UM2、SCC-4 和 SCC-9。用 TS 叶提取物处理这些细胞系，并筛选其活力、凋亡、坏死和凋亡基因表达。正常的人口腔角质形成细胞（NHOK）作为细胞毒性测定的对照。TS 处理的 HOSCC 的活力降低，而 NHOK 的活力没有受到影响。FACScan 分析显示，叶提取物根据细胞类型诱导凋亡或凋亡和坏死的组合。凋亡相关基因表达的微阵列和半定量 RT-PCR 分析表明，3,4,5-三羟基苯甲酸（没食子酸，从 TS 提取物中纯化的主要生物活性化合物之一）上调促凋亡基因，如 TNF-α、TP53BP2 和 GADD45A，并下调抗凋亡基因 Survivin 和 cIAP1，导致细胞死亡。本研究表明，存在的主要生物活性化合物没食子酸是香椿叶提取物抗肿瘤作用的原因。