Kira J, Koyanagi Y, Yamada T, Itoyama Y, Goto I, Yamamoto N, Sasaki H, Sakaki Y
Department of Neurology, Neurological Institute, Kyushu University, Fukuoka, Japan.
Ann Neurol. 1991 Feb;29(2):194-201. doi: 10.1002/ana.410290214.
Using the polymerase chain reaction, we quantitated the amount of human T-lymphotropic virus type I (HTLV-I) proviral DNA in peripheral blood mononuclear cells from 18 patients with HTLV-I--associated myelopathy/tropical spastic paraparesis; 17 HTLV-I carriers without HTLV-I--associated myelopathy/tropical spastic paraparesis, with or without other autoimmune or inflammatory diseases; and 19 seronegative control subjects. The HTLV-I proviral DNA was 10- to 100-fold higher in the patients and in the HTLV-I carriers without HAM/TSP who had autoimmune or inflammatory diseases than in the carriers without autoimmune or inflammatory diseases. The patients who had had onset of myelopathy at a younger age (15 to 39 years) had an extremely high level of HTLV-I proviral DNA in the early phase, as compared with findings in those with a late onset of myelopathy (at 44 to 61 years). The large increase in HTLV-I proviral DNA in peripheral blood mononuclear cells is presumably closely related to the development of autoimmune or inflammatory processes in HTLV-I carriers, including HTLV-I--associated myelopathy/tropical spastic paraparesis.
我们运用聚合酶链反应，对18例与人类嗜T淋巴细胞病毒I型（HTLV-I）相关的脊髓病/热带痉挛性截瘫患者、17例无HTLV-I相关脊髓病/热带痉挛性截瘫的HTLV-I携带者（无论有无其他自身免疫性或炎性疾病）以及19例血清学阴性对照者外周血单个核细胞中的HTLV-I前病毒DNA量进行了定量分析。与无自身免疫性或炎性疾病的携带者相比，患有自身免疫性或炎性疾病的患者及无HAM/TSP的HTLV-I携带者体内的HTLV-I前病毒DNA高出10至100倍。与脊髓病发病较晚（44至61岁）的患者相比，发病年龄较轻（15至39岁）的脊髓病患者在疾病早期HTLV-I前病毒DNA水平极高。外周血单个核细胞中HTLV-I前病毒DNA的大幅增加可能与HTLV-I携带者（包括HTLV-I相关脊髓病/热带痉挛性截瘫）自身免疫或炎性过程的发展密切相关。
