Department of Anatomy, Histology and Embryology, Semmelweis University, Tuzoltó 58, Budapest, H-1094, Hungary.
J Histochem Cytochem. 2010 May;58(5):463-79. doi: 10.1369/jhc.2010.954768. Epub 2010 Feb 1.
The so-called neurointermediate lobe is composed of the intermediate and neural lobes of the pituitary. The present immunohistochemical study investigated components of the basal lamina (laminin, agrin, and perlecan), the dystrophin-dystroglycan complex (dystrophin, beta-dystroglycan, alpha1-dystrobrevin, beta-dystrobrevin, utrophin, and alpha1-syntrophin), and the aquaporins (aquaporin-4 and -9). Glia markers (GFAP, S100, and glutamine synthetase) and components of connective tissue (collagen type I and fibronectin) were also labeled. In the neurohypophysis, immunostaining of basal lamina delineated meningeal invaginations. In these invaginations, vessels were seen to penetrate the organ without submerging into its parenchyma. On the parenchymal side of the invaginations, beta-dystroglycan was detected, whereas utrophin was detected in the walls of vessels. Immunostaining of alpha1-dystrobrevin and alpha1-syntrophin did not delineate the vessels. The cells of the intermediate lobe were fully immunoreactive to alpha1-dystrobrevin and alpha1-syntrophin, whereas components of the basal lamina delineated the contours of the cells. GFAP-immunoreactive processes surrounded them. Aquaporin-4 localized at the periphery of the neurohypophysis, mainly adjacent to the intermediate lobe but not along the vessels. It colocalized only partially with GFAP and not at all with alpha1-syntrophin. Aquaporin-9 was not detected. These results emphasize the possibility that the components of the dystrophin-dystroglycan complex localize differently and raise the question about the roles of dystrobrevins, alpha1-syntrophin, and aquaporin-4 in the functions of the intermediate and neural lobes, respectively.
所谓的神经垂体中间叶由垂体的中间叶和神经叶组成。本免疫组织化学研究调查了基膜的成分（层粘连蛋白、神经节苷脂和 perlecan）、营养不良肌聚糖复合物（营养不良蛋白、β-肌聚糖、α1-肌聚糖、β-肌聚糖、utrophin 和 α1-连接蛋白）和水通道蛋白（水通道蛋白-4 和 -9）。胶质细胞标志物（GFAP、S100 和谷氨酰胺合成酶）和结缔组织成分（胶原 I 型和纤维连接蛋白）也被标记。在神经垂体中，基膜的免疫染色勾勒出脑膜内陷。在这些内陷中，血管被看到穿透器官而不潜入其实质。在内陷的实质侧，检测到β-肌聚糖，而 utrophin 则检测到血管壁中。α1-肌聚糖和α1-连接蛋白的免疫染色没有勾勒出血管。中间叶的细胞对α1-肌聚糖和α1-连接蛋白完全具有免疫反应性，而基膜的成分勾勒出细胞的轮廓。GFAP 免疫反应性的过程围绕着它们。水通道蛋白-4 位于神经垂体的外周，主要邻近中间叶，但不沿血管分布。它仅部分与 GFAP 共定位，与α1-连接蛋白完全不共定位。未检测到水通道蛋白-9。这些结果强调了营养不良肌聚糖复合物的成分可能定位不同，并提出了关于肌聚糖、α1-连接蛋白和水通道蛋白-4 在中间叶和神经叶功能中的作用的问题。
