Larson Steven B, Day John S, Nguyen Chieugiang, Cudney Robert, McPherson Alexander
Department of Molecular Biology and Biochemistry, The University of California, Irvine, CA 92697-3900, USA.
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 Feb 1;66(Pt 2):113-20. doi: 10.1107/S174430910905194X. Epub 2010 Jan 26.
Bovine pancreatic ribonuclease A (RNase A) was crystallized from a mixture of small molecules containing basic fuchsin, tobramycin and uridine 5'-monophosphate (U5P). Solution of the crystal structure revealed that the enzyme was selectively bound to U5P, with the pyrimidine ring of U5P residing in the pyrimidine-binding site at Thr45. The structure was refined to an R factor of 0.197 and an R(free) of 0.253.
牛胰核糖核酸酶A(RNase A)是从含有碱性品红、妥布霉素和5'-单磷酸尿苷(U5P)的小分子混合物中结晶得到的。晶体结构解析表明,该酶选择性地与U5P结合,U5P的嘧啶环位于苏氨酸45处的嘧啶结合位点。该结构精修后的R因子为0.197,自由R因子为0.253。