来自罂粟的蒂巴因还原酶的结晶及初步X射线衍射分析
Crystallization and preliminary X-ray diffraction analysis of salutaridine reductase from the opium poppy Papaver somniferum.
作者信息
Higashi Yasuhiro, Smith Thomas J, Jez Joseph M, Kutchan Toni M
机构信息
Donald Danforth Plant Science Center, 975 North Warson Road, St Louis, Missouri 63132, USA.
出版信息
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 Feb 1;66(Pt 2):163-6. doi: 10.1107/S174430910904932X. Epub 2010 Jan 27.
Abstract
The opium poppy Papaver somniferum is the source of the narcotic analgesics morphine and codeine. Salutaridine reductase (SalR; EC 1.1.1.248) reduces the C-7 keto group of salutaridine to the C-7 (S)-hydroxyl group of salutaridinol in the biosynthetic pathway that leads to morphine in the opium poppy plant. P. somniferum SalR was overproduced in Escherichia coli and purified using cobalt-affinity and size-exclusion chromatography. Hexagonal crystals belonging to space group P6(4)22 or P6(2)22 were obtained using ammonium sulfate as precipitant and diffracted to a resolution of 1.9 A.
摘要
罂粟（Papaver somniferum）是麻醉性镇痛药吗啡和可待因的来源。在罂粟植物中通向吗啡的生物合成途径中，水杨醛苷还原酶（SalR；EC 1.1.1.248）将水杨醛苷的C-7酮基还原为水杨醛醇的C-7（S）-羟基。罂粟SalR在大肠杆菌中过量表达，并使用钴亲和色谱和尺寸排阻色谱进行纯化。以硫酸铵作为沉淀剂获得了属于空间群P6(4)22或P6(2)22的六方晶体，其衍射分辨率为1.9 Å。
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