Taubenberger J K, Cole D E, Raffeld M, Poplack D G, Jaffe E S, Medeiros L J
Division of Cancer Biology, Diagnosis, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892.
Arch Pathol Lab Med. 1991 Apr;115(4):338-42.
Monoclonal antibodies have been recently developed that react with antigens expressed on T and B lymphocytes in routinely processed, paraffin-embedded lymphoid tissues. In this study, we assessed bone marrow clot and/or core biopsy sections of 19 cases of acute lymphoblastic leukemia (ALL) using routinely decalcified, B5- or formalin-fixed, paraffin-embedded sections and a panel of monoclonal antibodies, including LN1, LN2, L26, Leu-22, UCHL-1, and LCA. Each case had been previously phenotyped using freshly obtained aspirate material and a standard immunophenotypic protocol. Our results demonstrate the utility of the LN2 antibody in differentiating between precursor B-cell (pre-B) and precursor T-cell ALL. The LN2 antibody stained 11 of 12 cases of pre-B ALL and did not react with any of the seven T-cell ALLs. The other antibodies tested were less helpful. The Leu-22 antibody stained both pre-B and T-cell ALLs, while the results with UCHL-1 revealed peculiar nuclear staining of pre-B and T-cell ALLs; this we attributed to processing artifact. The L26 antibody reacted with only one case of pre-B ALL (also CD20 antigen positive), while the LN1 antibody did not react with any pre-B ALLs. Neither L26 nor LN1 stained any cases of T-cell ALL. The LCA antibody stained in only four (21%) of 19 cases, two pre-B and two T-cell ALLs. The results also suggest that this panel of antibodies may be useful in differentiating ALL from mature B-cell and T-cell lymphomas involving the bone marrow.
最近已开发出单克隆抗体，可与常规处理的石蜡包埋淋巴组织中T和B淋巴细胞上表达的抗原发生反应。在本研究中，我们使用常规脱钙、B5或福尔马林固定的石蜡包埋切片以及一组单克隆抗体（包括LN1、LN2、L26、Leu-22、UCHL-1和LCA），对19例急性淋巴细胞白血病（ALL）的骨髓凝块和/或芯针活检切片进行了评估。每例病例之前已使用新鲜获取的抽吸物材料和标准免疫表型分析方案进行过表型分析。我们的结果表明，LN2抗体在区分前体B细胞（pre-B）和前体T细胞ALL方面具有实用性。LN2抗体对12例pre-B ALL中的11例进行了染色，而与7例T细胞ALL中的任何一例均无反应。所测试的其他抗体帮助较小。Leu-22抗体对pre-B和T细胞ALL均有染色，而UCHL-1的结果显示pre-B和T细胞ALL有特殊的核染色；我们将此归因于处理假象。L26抗体仅与1例pre-B ALL（也为CD20抗原阳性）发生反应，而LN1抗体与任何pre-B ALL均无反应。L26和LN1均未对任何T细胞ALL病例进行染色。LCA抗体仅在19例中的4例（21%）中染色，2例pre-B和2例T细胞ALL。结果还表明，这组抗体可能有助于将ALL与累及骨髓的成熟B细胞和T细胞淋巴瘤区分开来。
