Linder J, Ye Y, Armitage J O, Weisenburger D D
Department of Pathology, University of Nebraska Medical Center, Omaha.
Mod Pathol. 1988 Jan;1(1):29-34.
Traditional methods for the immunophenotypic analysis of the non-Hodgkin's lymphomas require fresh or snap-frozen tissue for flow cytometric or immunohistochemical studies. The monoclonal antibodies LN1, LN2, and L26 have been recently developed to recognize B-cell-specific antigens that survive routine tissue processing and paraffin embedding. In this study, the ability of these three antibodies to mark the neoplastic cells in 160 cases of paraffin-embedded non-Hodgkin's lymphoma relative to frozen section immunophenotype (42 T-cell, 118 B-cell), manner of fixation (B5 versus 10% buffered formalin), and histological subtype was examined. With B5-fixed tissue, the percentages of B-cell lymphoma marking with the antibodies were as follows: L26, 96.6%; LN1, 88.2%; LN2, 93.7%. With formalin-fixed tissue, the percentages of B-cell lymphoma reacting with the antibodies were: L26, 89.1%; LN1, 26.2%; LN2, 57.8%. Each of the antibodies marked a small percentage of paraffin-embedded T-cell lymphomas: L26, 4.7%; LN1, 4.7%; LN2, 7.1%. LN2, and to a lesser extent LN1, stained Reed-Sternberg cells, a feature not seen with L26. Nor did L26 mark nonlymphoid neoplasms, a feature previously reported with LN1 and LN2. Since a high percentage of B-cell lymphomas react with these antibodies and they are relatively specific for B-cells, they should prove highly useful for the evaluation of both diagnostic and experimental pathology specimens. L26 offers the distinct advantage of working well in both B5 and formalin-fixed tissues and seemingly not marking epithelial neoplasms.
非霍奇金淋巴瘤免疫表型分析的传统方法需要新鲜或速冻组织用于流式细胞术或免疫组织化学研究。单克隆抗体LN1、LN2和L26最近已被开发出来，用于识别在常规组织处理和石蜡包埋后仍能存活的B细胞特异性抗原。在本研究中，检测了这三种抗体相对于冰冻切片免疫表型（42例T细胞、118例B细胞）、固定方式（B5与10%缓冲福尔马林）和组织学亚型，在160例石蜡包埋的非霍奇金淋巴瘤中标记肿瘤细胞的能力。对于用B5固定的组织，用这些抗体标记的B细胞淋巴瘤的百分比分别为：L26，96.6%；LN1，88.2%；LN2，93.7%。对于用福尔马林固定的组织，与这些抗体反应的B细胞淋巴瘤的百分比分别为：L26，89.1%；LN1，26.2%；LN2，57.8%。每种抗体都标记了一小部分石蜡包埋的T细胞淋巴瘤：L26，4.7%；LN1，4.7%；LN2，7.1%。LN2以及在较小程度上LN1，可对里德-斯腾伯格细胞染色，这一特征L26未出现。L26也不会标记非淋巴样肿瘤，而LN1和LN2此前曾有过标记非淋巴样肿瘤的报道。由于高比例的B细胞淋巴瘤与这些抗体反应，且它们对B细胞具有相对特异性，因此它们在诊断和实验病理标本评估中应具有很高的实用性。L26的明显优势在于在B5固定组织和福尔马林固定组织中均表现良好，且似乎不会标记上皮性肿瘤。
