Optical Bioimaging Laboratory, Division of Bioengineering, National University of Singapore, 7 Engineering Drive 1,Singapore 117576, Singapore.
Opt Lett. 2010 Feb 1;35(3):447-9. doi: 10.1364/OL.35.000447.
Differential interference contrast (DIC) microscopy is an inherently qualitative phase-imaging technique. What is obtained is an image with mixed phase-gradient and amplitude information rather than a true linear mapping of actual optical path length (OPL) differences. Here we investigate an approach that combines the transport-of-intensity equation (TIE) with DIC microscopy, thus improving direct visual observation. There is little hardware modification and the computation is noniterative. Numerically solving for the propagation of light in a series of through-focus DIC images allows linear phase information in a single slice to be completely determined and restored from DIC intensity values.
微分干涉对比(DIC)显微镜是一种本质上的定性相位成像技术。得到的是一个混合了相位梯度和幅度信息的图像,而不是实际光程差(OPL)的真实线性映射。在这里,我们研究了一种将强度传输方程(TIE)与 DIC 显微镜相结合的方法,从而提高了直接观察效果。这种方法几乎不需要硬件修改,并且计算是非迭代的。通过对一系列聚焦 DIC 图像的光传播进行数值求解,可以完全确定并从 DIC 强度值中恢复单个切片中的线性相位信息。