使用微分干涉对比显微镜的线性相位成像

Linear phase imaging using differential interference contrast microscopy.

作者信息

Arnison M R, Larkin K G, Sheppard C J R, Smith N I, Cogswell C J

机构信息

Physical Optics Laboratory, School of Physics, University of Sydney, NSW 2006, Australia.

出版信息

J Microsc. 2004 Apr;214(Pt 1):7-12. doi: 10.1111/j.0022-2720.2004.01293.x.

DOI:10.1111/j.0022-2720.2004.01293.x

PMID:15049862

Abstract

We propose an extension to Nomarski differential interference contrast microscopy that enables isotropic linear phase imaging. The method combines phase shifting, two directions of shear and Fourier-space integration using a modified spiral phase transform. We simulated the method using a phantom object with spatially varying amplitude and phase. Simulated results show good agreement between the final phase image and the object phase, and demonstrate resistance to imaging noise.

摘要

我们提出了对诺马斯基微分干涉对比显微镜的一种扩展，它能够实现各向同性线性相位成像。该方法结合了相移、两个剪切方向以及使用改进的螺旋相位变换的傅里叶空间积分。我们使用具有空间变化幅度和相位的模拟物体对该方法进行了模拟。模拟结果表明最终相位图像与物体相位之间具有良好的一致性，并证明了对成像噪声的抗性。