Gausman H W, Allen W A, Escobar D E
Appl Opt. 1974 Jan 1;13(1):109-11. doi: 10.1364/AO.13.000109.
Air was replaced with media of higher refractive indices by vacuum infiltration in leaves of cucumber, blackeye pea, tomato, and string bean plants, and reflectance of noninfiltrated and infiltrated leaves was spectrophotometrically measured. Infiltrated leaves reflected less light than noninfiltrated leaves over the 500-2500-nm wavelength interval because cell wall-air interfaces were partly eliminated. Minimal reflectance should occur when the average refractive index of plant cell walls was matched by the infiltrating fluid. Although refractive indices that resulted in minimal reflectance differed among the four plant genera, an average value of 1.425 approximates the refractive index of plant cell walls for the four plant genera.
通过对黄瓜、黑眼豆、番茄和菜豆植株的叶片进行真空渗透，用具有较高折射率的介质取代空气，并通过分光光度法测量未渗透和渗透叶片的反射率。在500-2500纳米波长区间内，渗透叶片比未渗透叶片反射的光更少，因为细胞壁与空气的界面部分被消除了。当渗透液的平均折射率与植物细胞壁的平均折射率相匹配时，反射率应达到最小值。尽管导致最小反射率的折射率在这四个植物属中有所不同，但1.425的平均值近似于这四个植物属的植物细胞壁的折射率。
