Department of Obstetrics and Gynecology, Affiliated Hospital of Qingdao Medical College, Qingdao University, Qingdao, Shandong Province 266003, P.R. China.
Oncol Rep. 2010 Mar;23(3):795-9.
Vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) are two important angiogenic and prognostic factors for endometrial cancer. Estrogen is considered to be associated with increasing risk of endometrial adenocarcinoma. We investigated the expression of estrogen-responsive ring finger protein (Efp), VEGF and bFGF in endometrial cancer and correlated the results with clinicopathological features. We measured the expression of Efp, VEGF and bFGF in normal, hyperplastic and malignant endometrial tissues by quantitative RT-PCR, ELISA or Western blot analysis. The expression of Efp mRNA was significantly decreased in the groups of endometrial cancer (EC) in comparison with the groups of normal endometrium (NE) (P<0.05). Expression of VEGF mRNA in the groups of EC and atypical hyperplasia (AH) was significantly increased in comparison with the groups of NE (P<0.05). The expression of bFGF mRNA in groups of EC and AH was higher than that of NE groups (P<0.01 and P<0.05). There was positive relevance of bFGF expression and histologic grade and negative relevance of Efp expression and histologic grade. The expression of Efp, VEGF and bFGF protein was in agreement with the mRNA. The high expression of VEGF and bFGF indicate that both contribute in the angiogenesis of endometrial cancer. The regulation of Efp and bFGF expression during endometrial carcinogenesis suggests their potential utility as a prognostic biomarker.
血管内皮生长因子（VEGF）和碱性成纤维细胞生长因子（bFGF）是子宫内膜癌两种重要的血管生成和预后因子。雌激素被认为与子宫内膜腺癌风险增加有关。我们研究了雌激素反应性环指蛋白（Efp）、VEGF 和 bFGF 在子宫内膜癌中的表达，并将结果与临床病理特征相关联。我们通过定量 RT-PCR、ELISA 或 Western blot 分析测量了正常、增生和恶性子宫内膜组织中 Efp、VEGF 和 bFGF 的表达。与正常子宫内膜（NE）组相比，子宫内膜癌（EC）组的 Efp mRNA 表达明显降低（P<0.05）。EC 和非典型增生（AH）组的 VEGF mRNA 表达与 NE 组相比显著增加（P<0.05）。EC 和 AH 组的 bFGF mRNA 表达高于 NE 组（P<0.01 和 P<0.05）。bFGF 表达与组织学分级呈正相关，Efp 表达与组织学分级呈负相关。Efp、VEGF 和 bFGF 蛋白的表达与 mRNA 一致。VEGF 和 bFGF 的高表达表明它们都有助于子宫内膜癌的血管生成。Efp 和 bFGF 表达在子宫内膜癌发生过程中的调节表明它们可能作为预后生物标志物具有潜在的应用价值。
