Jiang Hongbing, Liu Laikui, Ye Jinhai, Liu Hualian, Xing Shuzhong, Wu Yunong
Department of Oral and Maxillofacial Surgery, School of Stomatology, Nanjing Medical University, Nanjing, 210029, Jiangsu, People's Republic of China.
J Cancer Res Clin Oncol. 2010 Sep;136(9):1295-302. doi: 10.1007/s00432-010-0780-4. Epub 2010 Feb 2.
The aims of the present study were to examine whether focal adhesion kinase (FAK) expression is correlated with cervical lymph node metastasis of tongue cancer and to investigate the roles of FAK in the process of cancer cell migration, invasion and anoikis resistance using the human tongue cancer cell line, Tca8113.
FAK expression was evaluated in 5 normal oral mucosa, 10 premalignant lesions, 80 primary tongue cancers and 41 lymph node metastases using anti-FAK immunohistochemistry. The migration, invasion and anoikis resistance of tongue cancer cells were evaluated using wound healing assays, invasion assays and anoikis induction. The effect of FAK inhibition was evaluated using RNA interference (RNAi).
In total, 55 of 80 primary tongue cancers (69%) showed high expression of FAK, and 25 of 80 tumors (31%) and all normal oral mucosa or premalignant lesions showed low expression of FAK. There was a significant correlation between FAK expression and the cervical lymph node metastasis of tongue cancer. Moreover, RNAi-mediated FAK reduction decreased tongue cancer cell migration, invasion and anoikis resistance.
These results suggest that FAK may serve as a marker of cervical lymph node metastasis of tongue cancer and that RNAi targeting FAK could serve as a potential therapeutic for the treatment of tongue cancer.
本研究旨在检测粘着斑激酶（FAK）表达是否与舌癌颈淋巴结转移相关，并利用人舌癌细胞系Tca8113研究FAK在癌细胞迁移、侵袭及失巢凋亡抵抗过程中的作用。
采用抗FAK免疫组化法评估5例正常口腔黏膜、10例癌前病变、80例原发性舌癌及41例淋巴结转移灶中FAK的表达。采用伤口愈合试验、侵袭试验及失巢凋亡诱导评估舌癌细胞的迁移、侵袭及失巢凋亡抵抗。采用RNA干扰（RNAi）评估FAK抑制的效果。
80例原发性舌癌中，55例（69%）显示FAK高表达，80例肿瘤中的25例（31%）以及所有正常口腔黏膜或癌前病变均显示FAK低表达。FAK表达与舌癌颈淋巴结转移之间存在显著相关性。此外，RNAi介导的FAK降低可减少舌癌细胞的迁移、侵袭及失巢凋亡抵抗。
这些结果表明，FAK可能作为舌癌颈淋巴结转移的标志物，靶向FAK的RNAi可作为治疗舌癌的潜在疗法。
