Faculty of Science and Letters, Department of Biology, University of Adiyaman, 02040, Adiyaman, Turkey.
Fish Physiol Biochem. 2010 Dec;36(4):1021-8. doi: 10.1007/s10695-010-9380-0. Epub 2010 Feb 3.
This study was conducted to characterize the alterations in reduced glutathione (GSH) level, catalase (CAT) activity and proteins electrophoretic patterns in response to sublethal copper (Cu) exposure in Cyprinus carpio and to determine whether these responses are related to Cu accumulation in gills, chosen as target tissue. Fish were exposed to 0.1 and 1.0 mg/l Cu for 10 and 20 days. There were increasing level of Cu in the gill with increasing concentrations of metal in the exposure medium, and with increasing duration of exposure. GSH level and CAT activity increased in fish exposed to 1.0 mg/l Cu for both exposure periods, while no change was detected at the lower Cu concentration. Electrophoretic patterns of gill proteins by sodium dodecyl sulphate gel electrophoresis (SDS-PAGE) consist of 25-, 26-, 30-, 44- and 48-kDA medium molecular weight proteins (MMP) for five bands and 64-, 72-, 90- and 101-kDA high molecular weight proteins (HMP) for four bands in both control and treatment groups. The levels of 25-, 26- and 30-kDA MMP and 72- and 90-kDA HMP increased in response to Cu exposure. The present study demonstrated that Cu caused stress in fish gills and an acclimation with induction of GSH, CAT, MMP and HMP, which were important in the protection against metal damage, was observed.
本研究旨在描述鲤鱼鳃组织中还原型谷胱甘肽（GSH）水平、过氧化氢酶（CAT）活性和蛋白质电泳图谱的变化，以应对亚致死浓度铜（Cu）暴露，并确定这些反应是否与鳃组织中 Cu 的积累有关。鱼被暴露在 0.1 和 1.0mg/l 的 Cu 中 10 和 20 天。随着暴露介质中金属浓度的增加和暴露时间的延长，Cu 在鳃中的含量也随之增加。在暴露于 1.0mg/l Cu 的鱼中，GSH 水平和 CAT 活性均升高，而在较低浓度的 Cu 下未检测到变化。通过十二烷基硫酸钠凝胶电泳（SDS-PAGE）对鳃蛋白的电泳图谱进行分析，对照组和处理组均有 25-、26-、30-、44-和 48-kDa 中分子量蛋白（MMP）的 5 条带和 64-、72-、90-和 101-kDa 高分子量蛋白（HMP）的 4 条带。Cu 暴露后，25-、26-和 30-kDa MMP 以及 72-和 90-kDa HMP 的水平增加。本研究表明，Cu 对鱼类鳃组织造成了应激，同时观察到 GSH、CAT、MMP 和 HMP 的诱导适应，这对于防止金属损伤非常重要。
