Department of Biology, Faculty of Sciences and Letters, University of Çukurova, 01330 Adana, Turkey.
Environ Toxicol Pharmacol. 2008 Jan;25(1):33-8. doi: 10.1016/j.etap.2007.08.007. Epub 2007 Aug 30.
In this study, levels of reduced glutathione (GSH) and metallothionein (MT) which are known to be biomarker of metal exposures were measured in a freshwater fish Oreochromis niloticus following exposure to 0, 5, 10 and 20μM concentrations of Cu, Zn, Cd and Pb for 14 days. Metals and GSH were measured in the liver, gill, intestine, muscle and blood, and MT in the liver. Copper accumulation occurred only in the gill, while Zn accumulation occurred only in the muscle. Lead accumulated in the liver and gill, whereas Cd accumulated in all the tissues. Metal exposures did not alter GSH levels in the blood, muscle and gill, but its levels increased in the liver following Cd, Zn and Cu exposures. MT levels in the liver increased only in Cd-exposed fish. The results showed that there was no significant change in tissue GSH levels following metal exposures, except in the liver. The levels of liver GSH increased significantly by all the metals, except lead. Data indicated that only the liver may be suitable indicator tissue to determine the response of GSH and MTs to metal exposure in environmental monitoring studies.
在这项研究中，我们测量了暴露于 0、5、10 和 20μM 浓度的 Cu、Zn、Cd 和 Pb 14 天后，一种淡水鱼奥利亚罗非鱼（Oreochromis niloticus）肝脏、鳃、肠、肌肉和血液中的还原型谷胱甘肽（GSH）和金属硫蛋白（MT）水平，而 MT 仅在肝脏中进行测量。铜仅在鳃中积累，而锌仅在肌肉中积累。铅在肝脏和鳃中积累，而镉则在所有组织中积累。金属暴露没有改变血液、肌肉和鳃中的 GSH 水平，但镉、锌和铜暴露后，肝脏中的 GSH 水平增加。肝脏中的 MT 水平仅在镉暴露的鱼中增加。结果表明，除肝脏外，金属暴露后组织 GSH 水平没有明显变化。所有金属除铅外，均显著增加了肝脏 GSH 水平。数据表明，只有肝脏可能是环境监测研究中确定 GSH 和 MT 对金属暴露反应的合适指示组织。
