Department of Analytical Chemistry, University of Pardubice, Pardubice, Czech Republic.
J Sep Sci. 2010 Mar;33(4-5):453-63. doi: 10.1002/jssc.200900699.
Retention characteristics of seven silica-based chromatographic columns (Bidentate C18, Silica-hydride, Spherisorb ODS-1, Resolve C18, XTerra C18, XBridge Phenyl and Blaze C8 Polydentate) were compared. The columns differ in the character of the support, including silica type B, hydrosilated silica, and hybrid silica gel with incorporated methyl groups, or ethylene bridges; further in the type of chemically bonded stationary phase, including C(18), C(8) and phenyl ligands, endcapped and non-endcapped with single point, bidentate and polydentate multi-point attachment to the adsorbent surface. Special attention was focused on hydrophobic and polar selectivity, silanol activity and anion-exclusion. The columns were compared and classified on the basis of the results of the classical column performance tests, retention of homologous n-alkylbenzenes over a broad composition range of aqueous-organic mobile phases and of naphthalene sulphonic acids in aqueous mobile phases without organic solvents.
比较了七种基于硅胶的色谱柱（双齿 C18、硅氢化物、Spherisorb ODS-1、Resolve C18、XTerra C18、XBridge Phenyl 和 Blaze C8 多齿）的保留特性。这些柱子在载体的性质上有所不同，包括硅胶 B、氢化硅胶和带有甲基或乙烯桥的杂化硅胶；进一步在化学键合固定相的类型上有所不同，包括 C(18)、C(8)和苯基配体，采用单点、双齿和多齿单点或多点连接到吸附剂表面进行端封和非端封。特别关注疏水性和极性选择性、硅醇活性和阴离子排斥。根据经典柱性能测试的结果，对这些柱子进行了比较和分类，包括在宽水-有机流动相组成范围内保留同系物 n-烷基苯和在不含有机溶剂的水相移动相中保留萘磺酸。
