David Victor, Petre Jana, Moldoveanu Serban C
Department of Analytical and Physical Chemistry, Faculty of Chemistry, University of Bucharest, Sos. Panduri, No. 90, Sector 5, 050663 Bucharest, Romania.
National Research and Development for Industrial Ecology-ECOIND, Drumul Podu Dambovitei Str., No. 71-73, 060652 Bucharest, Romania.
Molecules. 2025 May 6;30(9):2062. doi: 10.3390/molecules30092062.
The evaluation of the time spent by a solute exclusively in the mobile phase (dead time) is of fundamental interest for the interpretation of the retention data and obtainment of thermodynamic parameters for the HPLC process. This parameter depends on the volume occupied by the mobile phase and on the volume of the effective stationary phase from the HPLC column, and the measurement of these volumes poses a real challenge. This review discusses the evaluation of volumes of various phases involved in the retention process of solutes, which are related to the dead time, and the phase ratio for the separation. This paper attempts to cover as many points of view as possible regarding this topic in liquid chromatography, which is of importance for almost all separation mechanisms.
评估溶质仅在流动相中所花费的时间（死时间）对于解释保留数据和获取高效液相色谱（HPLC）过程的热力学参数至关重要。该参数取决于流动相所占的体积以及HPLC柱中有效固定相的体积，而这些体积的测量是一项真正的挑战。本综述讨论了溶质保留过程中涉及的与死时间相关的各相体积的评估以及分离的相比。本文试图涵盖液相色谱中关于该主题尽可能多的观点，这对于几乎所有的分离机制都很重要。
