Wu Sheng-Bing, Zhou Mei-Qi, Zhou Yi-Ping, Wang Ke-Ming, Wang Yue-Lan, Chen Ye-Nong, Cao Jian
Anhui Provincial Key Lab and Incubation Base of Acu-moxibustion Theoretical Foundation and Techniques, Anhui College of Chinese Medicine, Hefei 230038, China.
Zhen Ci Yan Jiu. 2009 Oct;34(5):315-8, 328.
To compare the effects of electroacupuncture (EA) of different acupoint groups on heart rate variability (HRV) and cerebral glutamate (Glu) and aspartic acid (Asp) contents in cerebrocardiac syndrome (CCS) rats.
A total of 52 Wistar rats were randomized into normal (n = 8), sham-operation (sham, n = 8), model (n = 12), Shuigou-Fengfu (GV 26-GV 16, n = 12), and Neiguan-Xinshu (PC 6-BL 15, n = 12) groups. CCS model was established by intracerebral (Caudate nucleus) injection of clostridiopeptidase (1 U/microl) plus heparin (7 U/microl). EA (2 Hz/100 Hz, 1-3 mA) was applied to GV 26-GV 16 and PC 6-BL 15 for 20 min, once daily for 3 days. Cerebral Glu and Asp contents were detected by high performance liquid chromatography, and HRV was analyzed by SMUP Software.
In comparison with normal and sham groups, the R-R interval (RRI) of electrocardiogram and total variability (TV) of HRV, cerebral Glu and Asp contents in model group increased significantly (P < 0.01, P < 0.05), and the ratio of low frequency/high frequency (LF/HF) decreased evidently (P < 0.01). Compared with model group, RRI and TV values, and cerebral Glu and Asp contents of EA of GV 26-GV 16 and PC 6-BL 15 groups reduced markedly (P < 0.01, P < 0.05), LF/HF of the later two groups increased evidently (P < 0.01). No significant differences were found between GV 26-GV 16 and PC 6-BL 15 groups in RRI, TV and LF/HF of HRV, and cerebral Glu and Asp contents (P > 0.05).
EA of both GV 26-GV 16 and PC 6-BL 15 can effectively improve CCS in rats, which is closely associated with their effects in lowering cerebral Glu and Asp levels and in suppressing the excitability of sympathetic nerve.
比较不同穴位组电针治疗对心脑综合征（CCS）大鼠心率变异性（HRV）及脑内谷氨酸（Glu）和天冬氨酸（Asp）含量的影响。
将52只Wistar大鼠随机分为正常组（n = 8）、假手术组（假手术，n = 8）、模型组（n = 12）、水沟-风府组（GV 26-GV 16，n = 12）和内关-心俞组（PC 6-BL 15，n = 12）。通过脑内（尾状核）注射梭菌肽酶（1 U/μl）加肝素（7 U/μl）建立CCS模型。将电针（2 Hz/100 Hz，1-3 mA）施加于GV 26-GV 16和PC 6-BL 15，持续20分钟，每天1次，共3天。采用高效液相色谱法检测脑内Glu和Asp含量，并用SMUP软件分析HRV。
与正常组和假手术组相比，模型组心电图R-R间期（RRI）、HRV总变异性（TV）、脑内Glu和Asp含量显著增加（P < 0.01，P < 0.05），低频/高频比值（LF/HF）明显降低（P < 0.01）。与模型组相比，GV 26-GV 16和PC 6-BL 15组电针治疗后的RRI和TV值以及脑内Glu和Asp含量显著降低（P < 0.01，P < 0.05），后两组的LF/HF明显增加（P < 0.01）。GV 26-GV 16组和PC 6-BL 15组在HRV的RRI、TV和LF/HF以及脑内Glu和Asp含量方面无显著差异（P > 0.05）。
GV 26-GV 16和PC 6-BL 15两组电针均能有效改善大鼠CCS，这与其降低脑内Glu和Asp水平以及抑制交感神经兴奋性的作用密切相关。
