Esfehani Y Javadi, Khavazi K, Ghorbani S
Department of Microbiology, Alzahra University, Vanak, Postal Code 1993891176, Tehran, Iran.
Pak J Biol Sci. 2009 Oct 15;12(20):1365-70. doi: 10.3923/pjbs.2009.1365.1370.
To study the cross interaction of microorganisms in plant growth promotion and the effect of auxin in this interaction, Pseudomonas putida and Glomus intraradices were co-inoculated on wheat. For this purpose, a mutant that produced less amount of auxin was derived from the bacterial strain by chemical mutagenesis with ethyl methane sulfonate. Next both the wild-type strain and the mutant strain were labeled with gusA for a better detection on the roots. Finally, the bacteria were inoculated on wheat as single inoculants or combined with arbuscular mycorrhizal fungi. Controls included non-bacteria/non-arbuscular mycorrhizal fungi and arbuscular mycorrhizal fungi alone. Plants co-inoculated with the wild-type bacterial strain and Glomus intraradices had the highest stem and their fresh and dry shoot weight was more than other treatments. Also, it was observed that the wild-type strain significantly improved the mycorrhizal colonization which implies the beneficial effect of auxin on mycorrhizal colonization. Furthermore, Glomus intraradices increased the population density of the wild-type strain in the endorhizosphere. In the microscopic studies of plant roots, bacterial colonies were observed as blues lines on the surface and inside the roots as well as on the seed surfaces.
为了研究微生物在促进植物生长中的交叉相互作用以及生长素在这种相互作用中的作用，将恶臭假单胞菌和根内球囊霉共同接种到小麦上。为此，通过用甲磺酸乙酯进行化学诱变，从该细菌菌株中获得了一种生长素产量较低的突变体。接下来，将野生型菌株和突变体菌株都用gusA标记，以便在根上进行更好的检测。最后，将细菌作为单一接种剂接种到小麦上，或与丛枝菌根真菌联合接种。对照包括非细菌/非丛枝菌根真菌和单独的丛枝菌根真菌。与野生型细菌菌株和根内球囊霉共同接种的植物茎最高，其地上部分鲜重和干重均高于其他处理。此外，观察到野生型菌株显著提高了菌根定殖率，这意味着生长素对菌根定殖有有益作用。此外，根内球囊霉增加了根内际野生型菌株的种群密度。在对植物根的显微镜研究中，细菌菌落在根表面、根内部以及种子表面均被观察为蓝色线条。
