眼科研究中使用的35毫米扫描仪的稳定性。
Stability of 35-mm scanners as used in ophthalmologic research.
作者信息
Jensen Karl, Lee Kristine E, Knudtson Michael D, Klein Ronald, Klein Barbara E K
机构信息
Department of Ophthalmology and Visual Sciences, University of Wisconsin School of Medicine and Public Health, Madison, Wisconsin 53726-2336, USA.
出版信息
Ophthalmic Surg Lasers Imaging. 2010 Jan-Feb;41(1):60-6. doi: 10.3928/15428877-20091230-11.
BACKGROUND AND OBJECTIVE
To assess the consistency of digitization of 35-mm slides as practiced in ophthalmologic research and estimate the impact of variation on semi-automated retinal vessel width measurements.
PATIENTS AND METHODS
A single retina slide was repeatedly digitized under various conditions on three scanner models. Average color levels were extracted from the resulting images, from which vessel widths were graded. The color channel level variations and possible correlation with width were analyzed.
RESULTS
The Nikon 5000 scanner (Nikon Corp., Tokyo, Japan) had average coefficients of variation of 0.4, 2.3, and 0.5 for the red, green, and blue channel levels across all runs. The P values of the correlation between the red, green, and blue color channel levels and the width of the large retinal arteriole were .89, .27, and .58, respectively.
CONCLUSION
The results suggest that the tested scanners digitize the 35-mm slides in a reliable manner without biasing the retinal vessel measurements.
背景与目的
评估眼科研究中35毫米幻灯片数字化的一致性，并估计变化对半自动视网膜血管宽度测量的影响。
患者与方法
在三种扫描仪型号的不同条件下，对一张视网膜幻灯片进行重复数字化。从所得图像中提取平均颜色水平，并据此对血管宽度进行分级。分析颜色通道水平变化及其与宽度的可能相关性。
结果
尼康5000扫描仪（尼康公司，东京，日本）在所有扫描过程中，红色、绿色和蓝色通道水平的平均变异系数分别为0.4、2.3和0.5。红色、绿色和蓝色颜色通道水平与视网膜大动脉宽度之间的相关性P值分别为0.89、0.27和0.58。
结论
结果表明，所测试的扫描仪能够以可靠的方式对35毫米幻灯片进行数字化，且不会对视网膜血管测量产生偏差。
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