Unidade de Tecnologias de Proteínas e Anticorpos Monoclonais, Instituto de Higiene e Medicina Tropical, Estrada do Paço do Lumiar 22, 1649-038 Lisboa, Portugal.
Parasitology. 2010 May;137(6):939-46. doi: 10.1017/S003118200999196X. Epub 2010 Feb 4.
Molecular detection of Babesia species in apparently healthy cattle within an endemic region was carried out in order to determine the prevalence of carriers and the geographical distribution of Babesia bigemina and Babesia bovis in Maputo Province, Mozambique. Samples from 477 animals at 5 localities were analysed using 2 techniques, the semi-nested hot-start PCR and the reverse line blot (RLB) assay. With the semi-nested hot-start PCR, detection of B. bigemina ranged between 30% and 89%, and of B. bovis between 27% and 83%. The RLB assay was comparatively less sensitive in this study and detection of B. bovis ranged from 0% to 17%, and B. bigemina was not detected at all by this technique. Analysis of new sequences of the 18S rRNA gene revealed that the current B. bigemina RLB probe is not specific for the identification of isolates in Mozambique. The RLB assay, however, resulted in the detection of 8 other haemoparasite species belonging to the genera Babesia, Theileria, Anaplasma and Ehrlichia. 18S rRNA gene sequences from the Theileria spp. were identified, and a phylogenic tree constructed with these sequences yielded a heterogeneous T. mutans-like group. In conclusion, infection with B. bigemina and B. bovis is endemic in Maputo Province, but rates of transmission vary. Furthermore, mixed infections with the haemoparasites responsible for several tick-borne diseases in cattle are common in Mozambique.
为了确定巴贝虫属物种在莫桑比克马普托省的带虫率和地理分布，对该地区看似健康的牛进行了巴贝虫属物种的分子检测，以确定带虫者的流行率。使用 2 种技术，即半巢式热启动 PCR 和反向线印迹（RLB）检测法，对来自 5 个地点的 477 个动物样本进行了分析。用半巢式热启动 PCR 检测，发现双芽巴贝斯虫的检出率为 30%至 89%，牛巴贝斯虫的检出率为 27%至 83%。在本研究中，RLB 检测法的敏感性相对较低，牛巴贝斯虫的检出率为 0%至 17%，且该技术未检测到双芽巴贝斯虫。对 18S rRNA 基因的新序列分析表明，目前的双芽巴贝斯虫 RLB 探针不能特异性鉴定莫桑比克的分离株。然而，RLB 检测法检测到了属于巴贝斯虫属、泰勒虫属、无形体属和埃立克体属的 8 种其他血液寄生虫。鉴定了泰勒虫属的 18S rRNA 基因序列，并构建了一个系统发育树，这些序列产生了一个异质的 T. mutans 样群。总之，马普托省巴贝虫属双芽巴贝斯虫和牛巴贝斯虫的感染呈地方性流行，但传播率不同。此外，莫桑比克牛的几种蜱传疾病的血液寄生虫混合感染很常见。
