安络化纤丸对大鼠实验性肝纤维化的抑制作用

[Inhibitory effect of anluohuaxianwan on experimental hepatic fibrosis in rats].

作者信息

Tan Xing-Hua, Li Chang-Qing, Zou Shang-Rong, Xie Min, Zhang Ai-Min, Li Wen-Li, Li Xiao-Yue, Huang Hui-Fen, Lei Chun-Liang

机构信息

Department of Liver Diseases, the Eighth People's Hospital of Guangzhou, Guangzhou 510060,China.

出版信息

Zhonghua Gan Zang Bing Za Zhi. 2010 Jan;18(1):9-12. doi: 10.3760/cma.j.issn.1007-3418.2010.01.003.

DOI:10.3760/cma.j.issn.1007-3418.2010.01.003

PMID:20128961

Abstract

OBJECTIVE

To investigate the effects of anluohuaqianwan on experimental hepatic fibrosis induced by dimethyl nitrosamine (DMN) in rats.

METHODS

36 male SD rats were randomly dividied into three groups: model group, normal group, anluohuaqianwan group. The rats in the three groups were treated with DMN daily for 4 weeks. The liver function was detected using auto biochemistry analyzer, the serum HA, LN, IV-C, PIIIP were detected by immunoradiometry, the histopathology was observed in the left liver lobe after HE staining, the expression of matrix metalloproteinase-2 (MMP-2) in liver tissue were detected by immunohistochemistry.

RESULTS

The serum levels of ALT, AST, ALP, TP, ALB and the contents of HA, LN, IV-C in model group were significantly increased compared to these in the normal group (P less than 0.01). The serum levels of ALT, AST and the contents of HA in anluohuaqianwan group were significantly lower than those in the model group (P less than 0.01). The liver MMP-2 in the model group was significantly increased compared to that in the normal group (P less than 0.05). The expression of MMP-2 in liver tissue of model group was lower than that in the anluohuaqianwan group (P less than 0.05).

CONCLUSION

Anluohuaqianwan can inhibit liver fibrosis in rats induced by DMN.

摘要

目的

探讨安络化纤丸对二甲基亚硝胺（DMN）诱导的大鼠实验性肝纤维化的影响。

方法

将36只雄性SD大鼠随机分为三组：模型组、正常组、安络化纤丸组。三组大鼠每日用DMN处理4周。使用自动生化分析仪检测肝功能，采用免疫放射法检测血清透明质酸（HA）、层粘连蛋白（LN）、Ⅳ型胶原（IV-C）、Ⅲ型前胶原肽（PIIIP），HE染色后观察左肝叶组织病理学变化，采用免疫组织化学法检测肝组织中基质金属蛋白酶-2（MMP-2）的表达。

结果

与正常组相比，模型组血清谷丙转氨酶（ALT）、谷草转氨酶（AST）、碱性磷酸酶（ALP）、总蛋白（TP）、白蛋白（ALB）水平及HA、LN、IV-C含量显著升高（P<0.01）。安络化纤丸组血清ALT、AST水平及HA含量显著低于模型组（P<0.01）。与正常组相比，模型组肝组织MMP-2显著升高（P<0.05）。模型组肝组织MMP-2表达低于安络化纤丸组（P<0.05）。