扶正降氨方对白蛋白诱导大鼠肝纤维化的影响

[Effect of Fuzheng Jiangan formula on liver fibrosis induced by albumin in rats].

作者信息

Huang Xiang-an, Sun Li-hong, Song Chong-shun, Cui Ning, Chen Yan-li, Xu Hong-yan, Ren Ying

机构信息

Department of Infectious Diseases, Dong Fang Hospital, Beijing University of Chinese Medicine, Beijing 100078, China.

出版信息

Zhongguo Zhong Yao Za Zhi. 2006 Nov;31(22):1890-3.

PMID:17285992

Abstract

OBJECTIVE

To observe the effect of Fuzheng Jiangan formula( FZJGF) on liver fibrosis using immune induced liver fibrosis rat model.

METHOD

The rat models with immunity liver fibrosis were induced by the human serum albumin. Rats were treated with normal saline, FZJGF (9. 85,39. 4 g x kg(-1) , two dosage groups) and Colchicine (0. 000 1 g . kg(-1) ). The activities of ALT, AST, contents of ALB and TP, and A/G, The contents of Laminin (LN), Hyaluronic acid (HA) and collagen type IV (IV-C) in rat serum were measured by radioimmunoassay method. The level of hydroxyproline (Hyp) in liver was detected by chemistry method. The pathological changes of liver tissue were observed by HE and Von-Gieson staining.

RESULT

FZJGF could significant decrease the serum activities of ALT and AST, and increase the levels of TP,Alb and ratio A/G. The levels of LN, HA and IV-C were decreased significantly after the treatment using FZJGF. The pathological improvements were observed. FZJGF could markedly alleviate the deposition of collageneous fiber, and reduce the liver pseudoluboli and the fibrosis scores in the liver tissue compared with model group.

CONCLUSION

FZJGF can inhibit formation and development of rat hepatic fibrosis induced by the human serum albumin.

摘要

目的

采用免疫诱导肝纤维化大鼠模型，观察扶正降肝方（FZJGF）对肝纤维化的影响。

方法

用人血清白蛋白诱导大鼠免疫性肝纤维化模型。大鼠分别用生理盐水、扶正降肝方（9.85、39.4 g·kg⁻¹，两个剂量组）和秋水仙碱（0.0001 g·kg⁻¹）进行治疗。采用放射免疫分析法测定大鼠血清中谷丙转氨酶（ALT）、谷草转氨酶（AST）活性，白蛋白（ALB）、总蛋白（TP）含量及白蛋白/球蛋白比值（A/G）。采用化学方法检测肝脏中羟脯氨酸（Hyp）水平。通过苏木精-伊红（HE）染色和冯·吉森（Von-Gieson）染色观察肝组织的病理变化。