School of Chinese Medicine, China Medical University, Taichung 404, Taiwan.
J Ethnopharmacol. 2011 Apr 12;134(3):953-60. doi: 10.1016/j.jep.2011.02.013. Epub 2011 Feb 17.
Yi Guan Jian (YGJ) has long been employed clinically to treat liver fibrosis in traditional Chinese Medicine but the mechanism underlying the regulation has not been clarified in detail. The present investigation was designed to assess the involvement of the fibrosis pathway in dimethylnitrosamine (DMN)-induced liver fibrosis in rats.
Liver fibrosis was induced by DMN injection (10mg/kg, i.p., given three consecutive days each week) following 4 weeks. YGJ was oral administered (1.8 g/kg daily via gastrogavage for two weeks). Liver sample were subjected to histological and western blot studies. For evaluation of hepatic fibrosis-related factors, collagen α1-I, tissue inhibitor of metalloproteinase-1 (TIMP-1), and α-smooth muscle actin (α-SMA) mRNA and protein levels were analyzed.
YGJ remarkably prevented body weight loss and DMN damage in the liver, and it inhibited the elevation of serum glutamate oxaloacetate transaminase (GOT), and glutamic pyruvic transaminase (GPT). Oral administration of YGJ extract significantly reduced the accumulation of collagen α1-I, TIMP-1, and α-SMA in liver tissues.
Taken together, these findings indicate that the YGJ Chinese herb showed hepatoprotective and anti-fibrogenic effects against DMN-induced hepatic injury. Our data suggest that the YGJ may be useful in reversing the development of hepatic fibrosis.
一贯煎（YGJ）长期以来一直被用于中医治疗肝纤维化，但对其调节机制尚未详细阐明。本研究旨在评估纤维化途径在二甲基亚硝胺（DMN）诱导的大鼠肝纤维化中的作用。
DMN 注射（10mg/kg，腹腔注射，每周连续 3 天）4 周后诱导肝纤维化。YGJ 经口给药（1.8g/kg，每日通过胃管给药，连续两周）。对肝组织进行组织学和 Western blot 研究。为了评估肝纤维化相关因子，分析胶原 α1-I、金属蛋白酶组织抑制剂-1（TIMP-1）和α-平滑肌肌动蛋白（α-SMA）的 mRNA 和蛋白水平。
YGJ 显著防止了体重减轻和 DMN 对肝脏的损害，并抑制了血清谷氨酸草酰乙酸转氨酶（GOT）和谷氨酸丙酮酸转氨酶（GPT）的升高。YGJ 提取物的口服给药显著减少了肝组织中胶原 α1-I、TIMP-1 和 α-SMA 的积累。
综上所述，这些发现表明 YGJ 中草药对 DMN 诱导的肝损伤具有保肝和抗纤维化作用。我们的数据表明，YGJ 可能有助于逆转肝纤维化的发展。
