[裸鼠模型中HCCLM3细胞与SMCC-7721细胞转移特性的比较]

[Comparison of the metastatic characteristics of HCCLM3 cells and SMCC-7721 cells in nude mice model].

作者信息

Li Jing-Dong, Peng Yong, Dai Yi, Li Bo, Wei Yong-Gang, Yan Lv-Nan

机构信息

Department of Hepatobiliary Surgery, Affiliated Hospital of North Sichuan Medical College, Sichuan Nanchong 637000, China.

出版信息

Zhonghua Gan Zang Bing Za Zhi. 2010 Jan;18(1):19-22. doi: 10.3760/cma.j.issn.1007-3418.2010.01.005.

DOI:10.3760/cma.j.issn.1007-3418.2010.01.005

PMID:20128963

Abstract

OBJECTIVE

To compare the metastatic characteristics of HCCLM3 cells and SMCC-7721 cells in nude mice model.

METHODS

Nude mice were divided into two groups (n = 8, each), mice were transplanted with HCCLM3 cells (group A) and SMMC-7721 cells (group B). Tumor size, metastasis rate and other clinical parameters were compared between the two groups. Statistical analysis was performed with the help of SPSS 16.0 for Windows computer software (SPSS, Inc., Chicago, IL). P values of less than 0.05 were considered statistically significant.

RESULTS

Intrahepatic metastases rate was 100% (8 / 8), mean intrahepatic primary tumor volume was (6954+/-1945) mm(3) in group A, Intrahepatic metastases rate was 62.5% (5/8), and mean intrahepatic primary tumor volume was (6034+/-2035) mm(3) in the group B. There was no statistical difference in the primary liver tumor size and intrahepatic metastases rate (P = 0.20; t = 6.38, P = 0.37, respectively). The numbers of intrahepatic metastases and the involved lobes, and the volume of tumor were 4.5 (median), 3, and 975 mm(3) (median) respectively, in group A, and these were 1 (median), 1 and 274 mm(3) (median) respectively in group B. The difference between two groups was statistically significant (Z values, -2.818, -2.289, and -1.975, respectively).The rate of lung metastasis and other organ metastasis in the A group was significantly higher than that in group B (P less than 0.001, P less than 0.041, respectively).

CONCLUSION

HCCLM3 cells have higher metastatic potential than SMMC-7721 cells in nude mice.

摘要

目的

比较HCCLM3细胞和SMCC - 7721细胞在裸鼠模型中的转移特性。

方法

将裸鼠分为两组（每组n = 8），分别移植HCCLM3细胞（A组）和SMMC - 7721细胞（B组）。比较两组的肿瘤大小、转移率及其他临床参数。借助Windows电脑软件SPSS 16.0（SPSS公司，伊利诺伊州芝加哥）进行统计分析。P值小于0.05被认为具有统计学意义。

结果

A组肝内转移率为100%（8/8），肝内原发肿瘤平均体积为(6954±1945)mm³；B组肝内转移率为62.5%（5/8），肝内原发肿瘤平均体积为(6034±2035)mm³。两组原发性肝肿瘤大小和肝内转移率无统计学差异（P = 0.20；t = 6.38，P = 0.37）。A组肝内转移灶数量及累及肝叶数、肿瘤体积分别为4.5（中位数）、3和975mm³（中位数），B组分别为1（中位数）、1和274mm³（中位数）。两组差异有统计学意义（Z值分别为 - 2.818、 - 2.289和 - 1.975）。A组肺转移率和其他器官转移率显著高于B组（P均小于0.001，P均小于0.041）。