Shandong University, Jinan, China.
J Biosci Bioeng. 2010 Jan;109(1):67-72. doi: 10.1016/j.jbiosc.2009.07.010. Epub 2009 Aug 15.
To test the effects of a sulfated polysaccharide, Haishen (HS) on the viability and proliferation of neural stem/progenitor cells (NSPCs), we isolated the polysaccharide from the body wall of the sea cucumber Stichopus japonicus by enzymolysis extraction, anion-exchange and gel-permeation chromatography. HS is a highly sulfated fucoidan with a molecular weight of 4.23x10(5) Da. Due to its safety being of invertebrate origin they are less likely to contain infectious agents, the effects of HS on the viability and proliferation of NSPCs in vitro were examined by MTT assay, BrdU labeling and neurosphere formation assay, respectively. Our results showed that HS alone increased NSPC viability in a dose-dependent manner. Moreover, HS acted synergistically with fibroblast growth factor-2 (FGF-2) but not epidermal growth factor (EGF) to enhance the proliferation of NSPCs. Finally, HS did not induce apoptosis of NSPCs. Our findings suggest that HS can serve as an adjuvant for promoting the proliferation of NSPCs.
为了测试硫酸化多糖海生（HS）对神经干细胞/祖细胞（NSPCs）活力和增殖的影响，我们通过酶解提取、阴离子交换和凝胶渗透色谱从海参体壁中分离出该多糖。HS 是一种高度硫酸化的岩藻聚糖，分子量为 4.23x10(5)Da。由于其来源于无脊椎动物，安全性更高，因此它们不太可能包含感染性物质。我们通过 MTT 检测、BrdU 标记和神经球形成检测分别研究了 HS 对 NSPCs 活力和增殖的影响。结果表明，HS 可单独剂量依赖性地增加 NSPC 活力。此外，HS 与成纤维细胞生长因子-2（FGF-2）协同作用，但与表皮生长因子（EGF）没有协同作用，可增强 NSPCs 的增殖。最后，HS 不会诱导 NSPCs 凋亡。我们的研究结果表明，HS 可作为促进 NSPCs 增殖的佐剂。
