Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, 2-24-16 Naka-cho, Koganei, Tokyo, Japan.
J Biosci Bioeng. 2010 Feb;109(2):202-4. doi: 10.1016/j.jbiosc.2009.07.011. Epub 2009 Aug 20.
We estimated the actual error rate during ligase detection reaction (LDR), and confirmed that DNA sequences around 3' ends are greatly influenced on the specificity of LDR. Its specificity is increased about 1000 times by introducing a mismatch base near the 3' ends.
我们估计了连接酶检测反应（LDR）过程中的实际错误率，并证实了 3' 末端附近的 DNA 序列对 LDR 的特异性有很大影响。在 3' 末端附近引入错配碱基可以将其特异性提高约 1000 倍。
