Ennis Madeleine
Respiratory Medicine Research Group, Centre for Infection and Immunity, Microbiology Building, The Queen's University of Belfast, Grosvenor Road, Belfast BT12 6BN, Northern Ireland, UK.
Homeopathy. 2010 Jan;99(1):51-6. doi: 10.1016/j.homp.2009.11.005.
This paper examines the activation and inhibition of activation of human basophils. After a brief description of human basophils, different methods to determine basophil activation are discussed with a special emphasis on the use of flow cytometric methods, as these circumvent the potential problems of assays based on the loss of colour by activated basophils. The activation of human basophils by ultra-high dilutions of anti-IgE is discussed. The majority of the paper describes the inhibition of basophil activation by ultra-high dilutions of histamine. The results from published papers are described and discussed. After over 20 years research trying to find out if high dilutions of histamine have a negative feedback effect on the activation of basophils by anti-IgE, what do we know? The methods are poorly standardized between laboratories - although the same is true for conventional studies. Certainly there appears to be some evidence for an effect - albeit small in some cases - with the high dilutions in several different laboratories using the flow cytometric methodologies. After standardization of a number of parameters, it is recommended that a multi-centre trial be performed to hopefully put an end to this "never-ending story".
本文研究了人类嗜碱性粒细胞的激活以及激活抑制情况。在对人类嗜碱性粒细胞进行简要描述之后，讨论了测定嗜碱性粒细胞激活的不同方法，特别强调了流式细胞术方法的应用，因为这些方法避免了基于激活的嗜碱性粒细胞颜色丧失的检测方法可能存在的问题。文中讨论了抗IgE超高稀释液对人类嗜碱性粒细胞的激活作用。本文大部分内容描述了组胺超高稀释液对嗜碱性粒细胞激活的抑制作用。对已发表论文的结果进行了描述和讨论。经过20多年试图弄清楚组胺高稀释液是否对抗IgE激活嗜碱性粒细胞具有负反馈作用的研究，我们了解到了什么呢？各实验室之间的方法标准化程度很低——尽管传统研究也是如此。当然，在几个不同实验室使用流式细胞术方法的情况下，似乎有一些证据表明高稀释液存在某种作用——尽管在某些情况下作用很小。在对一些参数进行标准化之后，建议开展一项多中心试验，以期结束这个“没完没了的故事”。
