基于 PCR 的伊朗南部自然感染的白蛉中华按蚊内脏利什曼原虫 kDNA 检测。
PCR-based detection of Leishmania major kDNA within naturally infected Phlebotomus papatasi in southern Iran.
机构信息
Department of Medical Entomology, Research Centre for Health Sciences, School of Health and Nutrition, Shiraz University of Medical Sciences, P.O. Box 71645-111, Shiraz, Iran.
出版信息
Trans R Soc Trop Med Hyg. 2010 Jun;104(6):440-2. doi: 10.1016/j.trstmh.2010.01.008. Epub 2010 Feb 2.
The annual incidence of cutaneous leishmaniasis (CL) in Iran rose by 43% over a five year period, from 2002 to 2006; most of these cases were caused by Leishmania major. Two complementary standard and nested polymerase chain reactions (PCRs) were used to detect parasites within their natural vector, Phlebotomus papatasi. Twelve different sand fly species were morphologically identified. The most abundant species (31.3%) was P. papatasi. Leptomonads were found in nine (2.4%) phlebotomines. Twenty (5.3%) sand fly species were found positive for Leishmania-genus DNA using standard PCR. The infection rate of this species was 5% and 7% by microscopic and molecular methods, respectively.
伊朗的皮肤利什曼病（CL）年发病率在 2002 年至 2006 年的五年间上升了 43%；其中大多数病例是由利什曼原虫引起的。两种互补的标准和巢式聚合酶链反应（PCR）用于检测其自然传播媒介白蛉中的寄生虫。鉴定出 12 种不同的沙蝇物种。最丰富的物种（31.3%）是白蛉属。在 9 种（2.4%）白蛉中发现了 Leptomonads。使用标准 PCR 发现 20 种（5.3%）沙蝇物种的利什曼属 DNA 呈阳性。该物种的感染率分别为 5%和 7%，通过显微镜和分子方法。
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