Shi H L, Furr H C, Olson J A
Department of Biochemistry and Biophysics, Iowa State University, Ames 50011.
Brain Res Bull. 1991 Feb;26(2):235-9. doi: 10.1016/0361-9230(91)90233-a.
The retinoids and carotenoids in bovine pineal gland were analyzed by high-performance liquid chromatographic methods. The major retinoid observed was all-trans retinol, with an average level of 579 pmol/g wet tissue. In agreement with a previous report, retinal was not detected; however, we could not detect any retinyl ester in bovine pineal gland. Methods similar to those used in studying metabolism of vitamin A in the bovine eye were used to study the esterification and isomerization reactions of retinoids in the pineal gland. Neither esterification nor isomerization reactions occurred. These results suggest that the key retinoids and enzymes involved in visual function are absent in the bovine pineal gland. The major carotenoid, which has not previously been reported in pineal gland, was beta-carotene at an average level of 1830 pmol/g wet tissue.
采用高效液相色谱法分析了牛松果体中的类视黄醇和类胡萝卜素。观察到的主要类视黄醇是全反式视黄醇，湿组织中的平均含量为579 pmol/g。与之前的一份报告一致，未检测到视黄醛；然而，我们在牛松果体中未检测到任何视黄醇酯。采用与研究牛眼中维生素A代谢相似的方法来研究松果体中类视黄醇的酯化和异构化反应。酯化和异构化反应均未发生。这些结果表明，牛松果体中不存在参与视觉功能的关键类视黄醇和酶。主要的类胡萝卜素是β-胡萝卜素，湿组织中的平均含量为1830 pmol/g，此前尚未在松果体中报道过。
